We narrowed to 14,443 results for: nts

1. pOPS0377

   Plasmid

   #115509

   Purpose

   Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Constitutive promoter pNeo pOGG001

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Cas9-NRRH

   Plasmid

   #136924

   Purpose

   Expresses Cas9-NRRH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9-NRRH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_Pro-IL-37

   Plasmid

   #214346

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL37 (IL37 Human)
   

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LAMP-CeNL_pcDNA3

   Plasmid

   #89514

   Purpose

   Cyan color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   CeNL
   

   Tags

   LAMP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fragment 1 Null

   Plasmid

   #198729

   Purpose

   Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   gRNA expression cassette in pENTR4
   

   Use

   Gateway-compatible entry vector

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. HBT_pcoCASphi_version1

   Plasmid

   #197931

   Purpose

   Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   FLAG tag and SV40 NLS and nucleoplasmin NLS

   Expression

   Plant

   Promoter

   35SPPDK promoter

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p35S-A3A-PBE

   Plasmid

   #119773

   Purpose

   Conversion of C to T in potato

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS-UGI-NLS
   

   Tags

   NLS (flanking UGI)

   Expression

   Plant

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   CaMv 2x35S

   Available Since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-SUN1_785

   Plasmid

   #125852

   Purpose

   Expresses SUN1_785 tagged with GFP in mammalian cells

   Depositor

   Miki Hieda

   Article

   Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

   Insert

   SUN1_785 (SUN1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMOD_C3003

   Plasmid

   #91095

   Purpose

   Module C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   GFP (+ BaeI site for GT donor cloning)
   

   Use

   Unspecified

   Promoter

   CmYLCV

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGM51559

    Plasmid

    #153226

    Purpose

    Control plasmid for knockout of TRY and CPC genes

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Cas9, guide RNA for AP3, FAST marker
    

    Use

    Synthetic Biology

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. VRER-ABEmax

    Plasmid

    #119812

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

    Insert

    ecTadA-ecTadA*(7.10)-nSpCas9 (VRER)
    

    Expression

    Mammalian

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD_A1510

    Plasmid

    #91036

    Purpose

    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-TaCas9
    

    Use

    CRISPR

    Promoter

    ZmUbi

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-UBC-MCP-mNeonGreen

    Plasmid

    #207581

    Purpose

    The lentiviral plasmids for expression of MCP-NeonGreen

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    MS2 coat protein mNeonGreen fusion
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Promoter

    UBC

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-ERK2 D319N

    Plasmid

    #8979

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D319N

    Available Since

    Oct. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pUb dPspCas13b + PspCas13b guide RNA

    Plasmid

    #176306

    Purpose

    Expresses catalytic dead PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Tags

    HA tag

    Expression

    Insect

    Mutation

    H133A, H1058A

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 HA Sam68 deltaKH

    Plasmid

    #17688

    Depositor

    David Shalloway

    Article

    Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

    Insert

    Sam68 (Khdrbs1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa 157-256 deleted (KH domain)

    Available Since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pCK364.306

    Plasmid

    #192642

    Purpose

    Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    BBa_J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

    Plasmid

    #200637

    Purpose

    Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    Vif
    

    Expression

    Mammalian

    Mutation

    T27stop

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMOD_A1910

    Plasmid

    #91041

    Purpose

    Module A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TREX2-P2A-TaCas9
    

    Use

    CRISPR

    Promoter

    ZmUbi

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJBL727

    Plasmid

    #140397

    Purpose

    Expresses CsoR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CsoR-T
    

    Tags

    6XHis-TEV

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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