19,647 results

1. Ki67-mNeonGreen-IRESpuro2

   Plasmid

   #183737

   Purpose

   Expresses Ki67-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. mScarlet-I-mTurquoise2

   Plasmid

   #98839

   Purpose

   Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mScarletI-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a-mCherry-MED1-IDR

   Plasmid

   #194545

   Purpose

   Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

   Insert

   MED1 (MED1 Human)
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_CD40_WT

   Plasmid

   #82198

   Purpose

   Gateway Donor vector containing CD40 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CD40 (CD40 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-6xGLY-3xFLAG-kanMX6

   Plasmid

   #20754

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Expression

   Yeast

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. mRuby2-N1

   Plasmid

   #54614

   Purpose

   Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Type

   Empty backbone

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV.CAG.Flex.GCaMP6f.WPRE.SV40

   Plasmid

   #100835

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV2, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CAG

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTag-RFP-C-h-Rab11a

   Plasmid

   #79806

   Purpose

   Express TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab11a (RAB11A Human)
   

   Tags

   TagRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. Flag-FOXA1

   Plasmid

   #153109

   Purpose

   Expression of Flag tagged human FOXA1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXA1 (FOXA1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mNeongreen-PXN

    Plasmid

    #129604

    Purpose

    In vivo visualization of focal adhesions (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Paxillin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-C1-PKCgamma-C1A

    Plasmid

    #21205

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKCgamma-C1A (Prkcg Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKCgamma-C1A domain. (Lab plasmid WC 0018)

    Available Since

    July 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA/Myc TCF4

    Plasmid

    #16512

    Depositor

    Bert Vogelstein

    Article

    Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

    Insert

    TCF4 (TCF7L2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCYC1m_yeGFP

    Plasmid

    #64389

    Purpose

    encodes yeast enhanced GFP under control of minimal pCYC1 promoter

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    yeast enhanced GFP
    

    Expression

    Yeast

    Promoter

    pCYC1

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Pgk

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmTurquoise2-T2A-Venus(L68V)

    Plasmid

    #60494

    Purpose

    Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2-T2A-mVenus(L68V)
    

    Tags

    T2A peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69545

    Purpose

    Tet-On system expressing GFP tagged with 2x nuclear localization signals.

    Depositor

    Charles Gersbach

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-RBD

    Plasmid

    #15247

    Depositor

    Martin Schwartz

    Article

    Ren et al EMBO J. 1999 Feb 1. 18(3):578-85.

    Insert

    Rhotekin (Rtkn Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 7-89 (Rho binding domain)

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-sgRNA Ef1alpha Puro-T2A-GFP

    Plasmid

    #111596

    Purpose

    sgRNA Ef1alpha Puro-T2A-GFP

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

    Insert

    mU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-beta-catenin deltaN90

    Plasmid

    #36985

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    beta-catenin deltaN90 (CTNNB1 Human)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 1-90 (Constitutively Acti…

    Promoter

    EF1a

    Available Since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEG BacMam C term 3C eGFP His8

    Plasmid

    #160687

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    3C eGFP His8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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