We narrowed to 22,191 results for: his

1. human p53-(1-393)-M340Q-L344R

   Plasmid

   #24863

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Methionine 340 mutated to Glutamine Leucine 344 …

   Promoter

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pUCBB-pBAD-eGFP

   Plasmid

   #32553

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   His

   Expression

   Mutation

   Promoter

   pBAD

   Available Since

   Oct. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCellFree_G03 TNF

   Plasmid

   #67062

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TNF (TNF Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)

   Plasmid

   #136546

   Purpose

   Expresses ANXA2 chimeras in bacterial cells

   Depositor

   Laszlo Nyitray

   Article

   Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

   Type

   Empty backbone

   Use

   Tags

   ANXA2 (23-339) and His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. EKAR2G_design1_mTFP_227_Venus_173

   Plasmid

   #39835

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pEF6 rglut1 HA complete

   Plasmid

   #89572

   Purpose

   rglut1 HA complete

   Depositor

   Jeffrey Rathmell

   Article

   Wieman et al Mol Biol Cell. 2007 Apr;18(4):1437-46. E

   Insert

   pEF6 rglut1 HA complete (Slc2a1 Rat)
   

   Use

   Tags

   V5-His A, B, C and rglut1 HA complete

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBR322-bom

   Plasmid

   #155178

   Purpose

   pBICI_2_strong without bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28 Set9 mutant

   Plasmid

   #24083

   Depositor

   Danny Reinberg

   Article

   Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

   Insert

   Set9 (SETD7 Human)
   

   Use

   Tags

   FLAG and His

   Expression

   Bacterial

   Mutation

   H297A

   Promoter

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pBAD-DiB2

   Plasmid

   #168801

   Purpose

   Fluorogen activating protein (FAP) DiB2

   Depositor

   Alexander Mishin

   Article

   Bozhanova et al Chem Sci. 2017 Oct 1;8(10):7138-7142. do

   Insert

   Fluorogen activating protein DiB2
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTorPE-Y-GECO2f

    Plasmid

    #105065

    Purpose

    A yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterial

    Depositor

    Robert Campbell

    Article

    Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

    Insert

    Y-GECO2f
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCellFree_G05

    Plasmid

    #67139

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His and mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA6-rbNHERF1

    Plasmid

    #32942

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    SLC9A3R1 (SLC9A3R1 rabbit)
    

    Use

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQTEV-MTPN

    Plasmid

    #34829

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    myotrophin (MTPN Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET15b-At 14-3-3 omega

    Plasmid

    #44930

    Depositor

    Robert Ferl

    Article

    Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

    Insert

    14-3-3 omega (GRF2 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pQTEV-STX10

    Plasmid

    #31572

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    syntaxin 10 (STX10 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCellFree_G06

    Plasmid

    #67140

    Purpose

    Gateway destination vector for cell-free expression with C-term mCherry 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    HIS and mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-GST-Q11

    Plasmid

    #185790

    Purpose

    Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    GST-Q11
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCellFree_G09

    Plasmid

    #67143

    Purpose

    Gateway destination vector for cell-free expression with N-term 8xHis sfGFP tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His and sfGFP

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-TALE-mCherry-15b

    Plasmid

    #138287

    Purpose

    Express tTALE-mCherry

    Depositor

    Kyubong Jo

    Article

    Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

    Insert

    7-mer tTALE-mCherry
    

    Use

    TALEN

    Tags

    6x His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. D. vulgaris Cas7/pHMGWA

    Plasmid

    #81184

    Purpose

    Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas7
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCellFree_G03 CTTN

    Plasmid

    #67100

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTTN (CTTN Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. PB_TRE_sg83-PDGFR_EF1a_iRFP670

    Plasmid

    #186311

    Purpose

    Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg83-PDGFR
    iRFP670
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PB_TRE_sg88-PDGFR_EF1a_eBFP2

    Plasmid

    #186312

    Purpose

    Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg88-PDGFR
    eBFP2
    

    Use

    Tags

    His, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-IP3KC

    Plasmid

    #32527

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2000 Dec 1;352 Pt 2:343-51.

    Insert

    IP3KC (ITPKC Human)
    

    Use

    Tags

    6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pET15b/EYFP-GgVcl 1-851

    Plasmid

    #46264

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-851 (VCL Chicken)
    

    Use

    Tags

    EYFP and His

    Expression

    Bacterial

    Mutation

    aa 1-851 (aka Vh) head domain

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET151-mUCH37 (ISF2)

    Plasmid

    #61928

    Purpose

    Expression of mUCH37 (ISF2) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    UCH37 (M.musculus) (Uchl5 Mouse)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EKAR2G_design1_mTFP_wt_Venus_157

    Plasmid

    #39814

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pDC146

    Plasmid

    #169366

    Purpose

    Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96

    Depositor

    Axel Brunger

    Article

    Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45

    Insert

    Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    

    Use

    Tags

    6 his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNC1137

    Plasmid

    #41563

    Depositor

    Beverly Errede

    Article

    Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

    Insert

    Ura3-FUS1-UBIMdkGFP*-SpHis5-Tim9
    

    Use

    Tags

    Expression

    Mutation

    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…

    Promoter

    Available Since

    April 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJC45BS104 Y819W

    Plasmid

    #1307

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 Y819W (HSP104 Budding Yeast)
    

    Use

    Tags

    10X His tag

    Expression

    Bacterial

    Mutation

    Changed Tyr 819 to Trp

    Promoter

    Available Since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCellFree_G03 GTF2B

    Plasmid

    #67067

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2B (GTF2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pET-s34T

    Plasmid

    #28256

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain H3-H4 with Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between H3 and H4

    Promoter

    Available Since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

33. pCellFree_G03 IFNG

    Plasmid

    #67056

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IFNG (IFNG Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pQTEV-SESN2

    Plasmid

    #34820

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    sestrin 2 (SESN2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EKAR2G_design2_mTFP_105_Venus_195

    Plasmid

    #40002

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pTorPE-Y-GECO1

    Plasmid

    #55765

    Purpose

    Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cells

    Depositor

    Robert Campbell

    Article

    Zhao et al Integr Biol (Camb). 2014 May 19.

    Insert

    Y-GECO1.0
    

    Use

    Lab constructed

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pQTEV-WDR41

    Plasmid

    #34808

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    WD repeat domain 41 (WDR41 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pK RagA-RagC

    Plasmid

    #99649

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    10x His tag, Poly arginine linker, and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. D. vulgaris Cas5c/pHMGWA

    Plasmid

    #81182

    Purpose

    Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas5c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. D. vulgaris Cas8c/pHMGWA

    Plasmid

    #81183

    Purpose

    Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Cas8c
    

    Use

    CRISPR

    Tags

    6xHis - MBP - TEV protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge