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BASIC_SEVA_17_pKD46.10

Plasmid

#178917

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

Feb. 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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PSICORhp-mp53

Plasmid

#26273

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

p53 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pGL1_4

Plasmid

#199036

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI plus NGS barcode 4

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD/HisD-LSSmKate1

Plasmid

#31908

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

LSSmKate1

Tags

HIS

Expression

Bacterial

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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p6VP1

Plasmid

#24725

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

VP1 from Human Polyomavirus 6

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pKEB31

Plasmid

#31224

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

ccdB and Cm resistance gene

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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p7VP1

Plasmid

#24726

Depositor

Christopher Buck

Article

Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

Insert

VP1 from Human Polyomavirus 7

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pPTK009-3a-αAmylase-αMFΔ

Plasmid

#84968

Purpose

Type 3a, α-Amylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-Amylase followed by MFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCM291rfp

Plasmid

#59775

Purpose

pCMrfp with TrfA mutation (291E to G)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pHLM

Plasmid

#64168

Purpose

harbours marker genes HIS3, LEU2 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pME-TagBFP-NS

Plasmid

#75342

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPTK010-3a-Glucoamylase-αMFΔ

Plasmid

#84969

Purpose

Type 3a, Glucoamylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glucoamylase followed by αMFΔ secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pGL0_4 [pIJ101 ORI]

Plasmid

#198921

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIJ101 ORI

Use

Synthetic Biology

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCZ886

Plasmid

#73584

Purpose

Pmex-5-HIS-72-miniSOG-3'UTR(tbb-2) for optogenetic mutagenesis

Depositor

Yishi Jin

Article

Noma et al Nat Commun. 2015 Dec 3;6:8868. doi: 10.1

Insert

HIS-72 (his-72 Nematode)

Use

For mossci insertion

Tags

miniSOG

Expression

Worm

Promoter

Pmex-5

Available Since

March 23, 2016

Availability

Academic Institutions and Nonprofits only

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XE246 Xenopus CK1 epsilon D to N CS2+

Plasmid

#16726

Depositor

Randall Moon

Article

Waxman et al Development. 2004 Dec. 131(23):5909-21.

Insert

casein kinase 1, epsilon (csnk1a1 Frog)

Use

Xenopus expression

Mutation

D128N

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pME-EGFP-NS

Plasmid

#75341

Purpose

Multisite gateway entry clone for adding N-terminal fusions of EGFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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LI C-D GUS

Plasmid

#104517

Purpose

LI Gene of Interest module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

UidA gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-i53

Plasmid

#119002

Purpose

Mammalian expression of i53 (53BP1) inhibitor and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

i53

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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OmpT

Plasmid

#213137

Purpose

OmpT_native membrane expression

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

OmpT

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pOZ-C 16 E7

Plasmid

#13705

Depositor

Karl Munger

Article

Huh et al Proc Natl Acad Sci U S A. 2005 Aug 9. 10

Insert

HPV 16 E7 (E7 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 12, 2007

Availability

Academic Institutions and Nonprofits only