We narrowed to 149,122 results for: ins;

1. pJL1 BSMT1

   Plasmid

   #106287

   Purpose

   Expresses BSMT1 in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   BSMT1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

   Plasmid

   #71811

   Purpose

   Generating transgenic flies for FLP-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
   

   Expression

   Insect

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLinker-AID-Ty-HXGPRT-LoxP

   Plasmid

   #86667

   Purpose

   for generation of AID strains using CRISPR tagging technology

   Depositor

   David Sibley

   Article

   Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

   Insert

   Linker-AID-Ty-HGPRT 3'UTR
   

   Use

   Expression in toxoplasma gondii

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MBP-His6-TEV-MMS2

   Plasmid

   #25465

   Depositor

   Cynthia Wolberger

   Article

   Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

   Insert

   MMS2 (MMS2, UBC13 Budding Yeast)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Available Since

   July 21, 2010

   Availability

   Academic Institutions and Nonprofits only

5. 1137=Hsp70Bb-Cas9[dsRed]

   Plasmid

   #149424

   Purpose

   The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Promoter

   Dmel Hsp70Bb

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

   Plasmid

   #67299

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   Tiam1-pMagFast2(3x)-iRFP (Tiam1 Mouse, Synthetic)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMyc[open]

   Plasmid

   #71809

   Purpose

   Generating transgenic flies for FLP-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   min.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
   

   Expression

   Insect

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. AICSDP-144:CTCF-mEGFP

   Plasmid

   #168799

   Purpose

   Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF

   Depositor

   Allen Institute for Cell Science

   Insert

   CTCF (CTCF Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   June 10, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pAct:dCas9-VP64

   Plasmid

   #78901

   Purpose

   Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS AluYa5rescueA70D

    Plasmid

    #78657

    Purpose

    Generates Alu inserts suitable for rescue

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

    Insert

    Ya5rescue cassette
    

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. NpT7-5-Erk2

    Plasmid

    #39230

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    Erk2 (Mapk1 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

12. T5-sgRNA

    Plasmid

    #113655

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter T5 and sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV2_VP1_RaPID_r1c3

    Plasmid

    #65723

    Purpose

    Produce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 only

    Depositor

    Junghae Suh

    Article

    Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

    Insert

    mCherry
    

    Use

    AAV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pKar2-msGFP-HDEL

    Plasmid

    #117668

    Purpose

    Localisation of Endoplasmic Reticulum

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pKar2-msGFP-HDEL
    

    Expression

    Yeast

    Mutation

    Contains the Kar2 promoter and signal sequence fr…

    Promoter

    pKar2

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-link-ymVenus-URA3

    Plasmid

    #168058

    Purpose

    Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymVenus
    

    Tags

    ymVenus

    Expression

    Yeast

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCOLA kiwi sensor sfGFP

    Plasmid

    #107368

    Purpose

    Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    kiwi-detecting toehold + sfGFP
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. cacnb1b (rat) in pMT2 vector

    Plasmid

    #107423

    Purpose

    Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

    Insert

    calcium channel beta1b auxillary subunit
    

    Expression

    Mammalian

    Promoter

    Ad MLP

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3xFRB-myc

    Plasmid

    #20226

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    3xFRB (MTOR Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pCIS-krPAM2

    Plasmid

    #25457

    Depositor

    Richard Mains

    Article

    Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

    Insert

    PAM2 (Pam Rat)
    

    Expression

    Mammalian

    Mutation

    Exon A deleted (exon A extends from nt 1475 to n…

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only