We narrowed to 6,356 results for: sas

1. pSIN-TRE-SspB-mCh-p60

   Plasmid

   #190170

   Purpose

   Doxycycline-inducible lentivirus vector to express SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   SspBmicro-mCherry-p60
   

   Use

   Lentiviral

   Tags

   SspBmicro-mCherry

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCutamp

   Plasmid

   #140632

   Purpose

   Plasmid-curing in Escherichia coli by targeting the AmpR promoter

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFX-mKate2-Rab11

   Plasmid

   #174463

   Purpose

   Express ｍKate2 with recycling endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mKate2 with recycling endosome-targeting sequence
   

   Use

   Tags

   small GTPase (Rab11)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTXB1-Tn5-F

   Plasmid

   #160081

   Purpose

   Bacterial expression of C-terminally FLAG-tagged Tn5 transposase

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase
   

   Use

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNW-sfGFP(SPS6)

   Plasmid

   #133814

   Purpose

   Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(SPS6)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppta

   Available since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS26_QUAS_CsChrimson

   Plasmid

   #175548

   Purpose

   Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

   Depositor

   Leslie Vosshall

   Article

   Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

   Insert

   CsChrimson
   

   Use

   Tags

   tdTomato

   Expression

   Insect

   Mutation

   Promoter

   QUAS

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFX-β-tubulin-EGFP

   Plasmid

   #175827

   Purpose

   Express EGFP with microtubule-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with microtuble-targeting sequence
   

   Use

   Tags

   human tubulin beta 4B class IVb;TUBB4B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSL3402 (pTnsABf_Pse)

   Plasmid

   #200901

   Purpose

   Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseTnsA-TnsB
   

   Use

   Tags

   Internal NLS between TnsA-TnsB fusion

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. TaRGET-ABE-C3.1 ver4.1

   Plasmid

   #188251

   Purpose

   Hypercompact adenine base editors

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Chem Biol. 2022 Sep;18(9):1005-1013.

   Insert

   TaRGET-ABE3.1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-ATBF1

    Plasmid

    #40927

    Depositor

    Jin-Tang Dong

    Article

    Dong et al J Biol Chem. 2010 Oct 22;285(43):32801-9

    Insert

    ATBF1 (ZFHX3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    CMV

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFX-mito-eqFP650

    Plasmid

    #174179

    Purpose

    Express eqFP650 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    eqFP650 with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSL0341 (pTarget)

    Plasmid

    #200906

    Purpose

    Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    pTarget
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_FLAG-Mma PylRS

    Plasmid

    #154762

    Purpose

    plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGFP1-mito

    Plasmid

    #174180

    Purpose

    Express AcGFP1 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    AcGFP with mitochondria-targeting sequence
    

    Use

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO-shPKM2_4

    Plasmid

    #42516

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    shRNA-PKM2 (PKM Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFX-EGFP-Sec61β

    Plasmid

    #174466

    Purpose

    Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with endoplasmic reticulum-targeting sequence
    

    Use

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV/Guide-mCherry

    Plasmid

    #170543

    Purpose

    Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS

    Plasmid

    #140008

    Purpose

    Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    4xPylT (Mma) cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    U25C

    Promoter

    h7SK

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL2783 (pTnsC-VP64_Vch)

    Plasmid

    #200896

    Purpose

    Expresses human codon-optimized V. cholerae TnsC-VP64 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchTnsC-VP64
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCD5-H5_Vietnam_1203/04_sfGFP_ST

    Plasmid

    #182546

    Purpose

    Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus

    Depositor

    Robert de Vries

    Article

    Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

    Insert

    H5_Vietnam_1203_04
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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