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pDONR221 Mak16

Plasmid

#63762

Purpose

yeast ORF Mak16 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Mak16

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221 Nip7

Plasmid

#63763

Purpose

yeast ORF Nip7 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Nip7

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Nog2

Plasmid

#63764

Purpose

yeast ORF Nog2 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Nog2

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Nop16

Plasmid

#63765

Purpose

yeast ORF Nop16 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Nop16

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Nop2

Plasmid

#63766

Purpose

yeast ORF Nop2 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Nop2

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Nop4

Plasmid

#63767

Purpose

yeast ORF Nop4 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Nop4

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Nsa2

Plasmid

#63768

Purpose

yeast ORF Nsa2 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Nsa2

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Nsr1

Plasmid

#63769

Purpose

yeast ORF Nsr1 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Nsr1

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Puf6

Plasmid

#63770

Purpose

yeast ORF Puf6 in the Gateway entry vector pDONR221

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Insert

Puf6

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Lasso 1

Plasmid

#69935

Purpose

The first of the two RNA lassos required for the RNA chain construction

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

TAATACGACTCACTATAGGG

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Lasso 2

Plasmid

#69936

Purpose

The second of the two RNA lassos required for the RNA chain construction.

Depositor

Alfonso Jaramillo

Article

Hassall et al Methods Mol Biol. 2015;1316:149-67. doi:

Insert

Lasso 2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

TAATACGACTCACTATAGGG

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pJK501

Plasmid

#72246

Purpose

Produces E. coli N5-carboxyaminoimidazole ribonucleotide mutase, Trp151>Phe mutant (EcPurE1-W151F), and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

Depositor

T. J. Kappock

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Use

Tags

Expression

Bacterial

Mutation

Gln206 to Arg and changes tryptophan-151 to pheny…

Promoter

T7 and endogenous

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E146 Hs.NRAS-nostop

Plasmid

#70430

Purpose

Gateway ORF clone of human NRAS [NM_002524.4] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

NRAS (NRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E104 Hs.ICMT-nostop

Plasmid

#70388

Purpose

Gateway ORF clone of human ICMT [NM_012405.3] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

ICMT (ICMT Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E078 Hs.EXOC6-nostop

Plasmid

#70362

Purpose

Gateway ORF clone of human EXOC6 [NM_019053.4] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

EXOC6 (EXOC6 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGGEV_3_FRT_+1_OA-

Plasmid

#49311

Purpose

Golden Gate entry vector: Position 3, FRT site

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Insert

FRT forward

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pGGEV_+1_XcmI-LacZ

Plasmid

#49295

Purpose

Golden Gate entry vector at position 1, plus 5' ATG, LacZ

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pLG173

Plasmid

#124368

Purpose

Control plasmid with only spectinomycin

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

empty backbone (control) (NEWENTRY Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

No promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pYPQ131B

Plasmid

#69281

Purpose

Golden Gate entry vector; 1st gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132B

Plasmid

#69282

Purpose

Golden Gate entry vector; 2nd gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge