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Showing: 691 - 720 of 846 results

  2. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid
    #176149
    Purpose
    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    X-ray repair cross complementing 1 (XRCC1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…
    Promoter
    CMV
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176149-plasmid-map-sequence-id-353190

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  3. GP805

    Plasmid
    #164545
    Purpose
    Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R
    Depositor
    Gregory Pazour
    Article
    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Insert
    Smoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    All 21 cytoplamic Lysines of Smoothened changed t…
    Promoter
    GgCryD1
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164545-plasmid-map-sequence-id-325341

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  9. pLenti_EF1α-NES-Kinprola_on-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233373
    Purpose
    EF1α driven co-expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_on-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233373-plasmid-map-sequence-id-466778

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  10. pLenti_EF1α-NES-Kinprola_PKA_T/A-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233372
    Purpose
    EF1α driven co-expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Inserts
    NES-Kinprola_PKA_T/A-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Expression
    Mutation
    Promoter
    EF1α
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233372-plasmid-map-sequence-id-466777

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  11. pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187611
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187611-plasmid-map-sequence-id-372189

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  12. pLV-EF1A-WWE(R163A)-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro

    Plasmid
    #187612
    Purpose
    Split luciferase construct with nLuc fused to C-terminus of WWE domain with Arg163Ala mutation, linked by T2A to cLuc fused to C-terminus of WWE domain, linked by IRES to puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii
    Inserts
    WWE domain (R163A) with C-terminal nLuc
    WWE domain with C-terminal cLuc
    Use
    Lentiviral
    Tags
    FLAG, HA, Split luciferase (cLuc), Split lucifera…
    Expression
    Mammalian
    Mutation
    Arg163Ala
    Promoter
    EF1A
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187612-plasmid-map-sequence-id-372035

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  20. pLV-CMV-EGFP-PolB(K72A)-PAMmut-Hygro

    Plasmid
    #176087
    Purpose
    EGFP fused to the N-terminus of POLB containing the mutation Lys72Ala, a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Lys72 to Ala, and mutation in the PAM…
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    176087-plasmid-map-sequence-id-352865

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  25. pLX_EFS-Cre_ppt-del

    Plasmid
    #162073
    Purpose
    Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Insert
    Cre
    Use
    Cre/Lox and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EFS (part of insert)
    Available since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162073-plasmid-map-sequence-id-325292

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  30. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

    Plasmid
    #216156
    Purpose
    Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216156-plasmid-map-sequence-id-437768

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Showing: 691 - 720 of 846 results