We narrowed to 1,368 results for: Flp
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Plasmid#155017PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to attenuated diphtheria toxin (tox176) in the presence of Cre and (r)tTA activityDepositorInsertDTA
UseMouse TargetingTagsExpressionMutationtox176PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FDIO-COX4-dAPEX2
Plasmid#117178PurposePlasmid name in publication: pAAV-FDIO-Matrix-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertCOX4-dAPEX2
UseAAV; Flp/frtTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FDIO-LACTB-dAPEX2
Plasmid#117181PurposePlasmid name in publication: pAAV-FDIO-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertLACTB-dAPEX2
UseAAV; Flp/frtTagsExpressionMammalianMutationW41F and A134P on soybean APXPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
BCL9-BirA*
Plasmid#109147PurposeTo generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorInsertBCL9 (BCL9 Human)
UseTagsBirA(R118G)ExpressionMammalianMutationPromoterCMV/TetO2Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT344_pattB-pCA-GFP-pA-FRT5
Plasmid#52550Purposefor Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing miceDepositorInsertsPhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO-EGFP-G3BP1-S149A
Plasmid#136004PurposeDOX-inducible S149A G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertG3BP1 (G3BP1 Human)
UseTagsEGFPExpressionMammalianMutationS149APromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FDIO-SYP-HRP
Plasmid#117187PurposePlasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labelingDepositorInsertSYP-HRP
UseAAV; Flp/frtTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1997_pExpr-hU6-CRISPR-DECODER
Plasmid#87549PurposeExpresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinasesDepositorInsertgRNAs targeting NGN2, MIAT, ACTC1, TTN
UseCRISPR, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
EW428 CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deImmLink-ZIK1KRAB
Plasmid#236141PurposePlasmid encoding the ZNF35/ZNF35 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-ZNF35(5-8)-deImmunLink-ZIK1KRAB
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW378 (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-GFP
Plasmid#236134PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with four ZNF35/ZNF250 bindi…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW408 (ZNF35tar-compact)x8-H2B-GFP
Plasmid#236129PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight ZNF35 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with eight ZNF35 binding sit…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW407 (ZNF35/ZNF35-6bp spacers)x4-H2B-GFP
Plasmid#236137PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with four ZNF35/ZNF250 bindi…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
SCN1Aprom(1-500)-H2B-GFP
Plasmid#236162PurposeFluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 500 bp upstream of its transcriptional start siteDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with the SCN1a promoter regi…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
SCN1Aprom(335-406)-H2B-GFP
Plasmid#236151PurposeFluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start siteDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with the SCN1a promoter regi…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW387 (ZNF35/IKZF1tar-6bp spacers)x4-H2B-GFP
Plasmid#236138PurposeFuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNFIKZF1 binding sites upstreamDepositorInsertH2B (H2BC11 Human)
UseTagsEGFPExpressionMammalianMutationPromoterE1b minimal promoter with four ZNF35/ZNFIKZF1 bin…Available sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW416 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmLink-ZIK1KRAB
Plasmid#236140PurposePlasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-ZNF250(1-4)-deImmunLink-ZIK1KRAB
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW425 CMV-TO-ZNF35(5-8)-IKZF1(2-3)-realDeImmLink-ZIK1KRAB
Plasmid#236142PurposePlasmid encoding the ZNF35/IKZF1 zinc finger array attached by a deimmunized linker to the ZIK1 KRAB domain, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-IKZF1(2-3)-deImmunLink-ZIK1KRAB
UseTagsExpressionMammalianMutationPromoterCMV promoter with two TetR binding sitesAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ093 SFFV-sensor-IRESv0-LIDARst(IRESv0-AVPR2)
Plasmid#233430PurposeLIDAR all components on single transcript (as shown in Figure 4A), using EMCV IRES to separate each componentDepositorInsertsensor-IRESv0-LIDARst(IRESv0-AVPR2)
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterSFFVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ098 SFFV-mCherry-LIDARsensor(W)-hIgG1-TM
Plasmid#233429PurposePositive control LIDAR reporter RNA (UAG stop codon replaced with UGG) with a membrane-bound human IgG as outputDepositorInsertmCherry-LIDARsensor(W)-hIgG1-TM
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
LSM041-3 SFFV-mCherry-SGc(stem-1(3xPP7))cSG-EGFP
Plasmid#233408PurposeLIDAR reporter RNA with PCP binding site instead of MCP; mCherry as marker, EGFP as outputDepositorInsertmCherry-SGc(stem-1(3xPP7))cSG-EGFP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ392 SFFV-secNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP
Plasmid#233415PurposeLIDAR reporter RNA; secreted NanoLuc Luciferase as marker, SEAP as outputDepositorInsertsecNLuc-SGc(stem-1(3xMS2))cSG(tight)-ssSEAP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ400 SFFV-mCherry-SGc(stem-1(3xMS2))cSG-TEVP (equivalent to EK0461)
Plasmid#233414PurposeLIDAR reporter RNA; mCherry as marker, TEVP as output (equivalent to EK0461)DepositorInsertmCherry-SGc(stem-1(3xMS2))cSG-TEVP
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
APL0038 Ac5-mCherry-SGc(stem-1(3xMS2))cSG-EGFP
Plasmid#233410PurposeAC5 promoter driven LIDAR reporter RNA for drosophila cell expression (mCherry as marker, EGFP as output)DepositorInsertmCherry-SGc(stem-1(3xMS2))cSG-EGFP
UseTagsExpressionMammalianMutationWTPromoterAc5Available sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
YH039 PGK-TO-FKBP-tm-GS-XTEN-ADAR2(DD-E488Q-T501A)
Plasmid#233378PurposePGK promoter driven FKBP fused with ADAR2dd (E488Q,T501A), with a CD28 transmembrane domain in betweenDepositorInsertFKBP-ADAR2dd
UseTagsExpressionMammalianMutationE488Q, T501A on ADAR2ddPromoterPGKAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C+F-ArchT-GFP
Plasmid#209700PurposeExpresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinasesDepositorInsertArchT-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP
Plasmid#205603PurposeTet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinWT-RNAi-resistant-GFP
UseTagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240APromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-fDIO-tdTomato-WPRE
Plasmid#187112PurposeFLP-dependent expression of tdTomato under EF1a promoterDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV1524
Plasmid#111431PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry
Plasmid#75470PurposeFlp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertReversed ChR2(H134R)-mCherry
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP
Plasmid#90008PurposeSplit-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-FRT hM4Di-mCherry
Plasmid#161576PurposeExpression of hM4Di-mCherry in a Flp-dependent fashionDepositorInserthM4Di-mCherry
UseAAVTagsExpressionMutationNAPromoterhSynAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_FKBP-Myc-Nbir
Plasmid#90004PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and C-terminally tagged FKBP-NBir[2-256]DepositorInsertsFKBP-linker-Myc-NBirA*
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-mNeonGreen
Plasmid#99133PurposeAn AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoterDepositorHas ServiceAAV1InsertfDIO-mNeonGreen
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-CBir-FRB_Myc-NBir-FKBP
Plasmid#90003PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBPDepositorInsertsNBirA*-linker-FKBP
CBirA*-linker-FRB
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Sstr3-GFP-DEST
Plasmid#49098PurposeExpressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSstr3 (Sstr3 Mouse)
UseTagsGFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-FRB-Cbir_FKBP-Myc-Nbir
Plasmid#90009PurposeSplit-BioID plasmid: Expresses C-terminally tagged CBir[257-321]-FRB and C-terminally tagged NBir[2-256]-FKBPDepositorInsertsFKBP-linker-Myc-NBirA*
FRB-linker-CBirA*
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn1-fDIO-mScarlet-KASH-2a-dCre
Plasmid#203839PurposeFlp-dependent enzymatically inactive Cre and mScarlet-KASH expressionDepositorInsertdCre
UseAAVTagsmScarlet-KASH-2AExpressionMutationEnzymatically inactive CrePromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TO-DEST
Plasmid#106348PurposeDestination vector for gateway cloning. Developed for Flp-In T-REx system.DepositorTypeEmpty backboneUseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx-FRT hM3Dq-mCherry
Plasmid#161575PurposeExpression of hM3Dq-mCherry in a Flp-dependent fashionDepositorInserthM3Dq-mCherry
UseAAVTagsExpressionMutationNAPromoterhSynAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-VARNAM2-Kv2.1PR
Plasmid#195536PurposeFlp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationVARNAM SI linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only