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We narrowed to 2,180 results for: Pam

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Showing: 921 - 960 of 2180 results

  1. NG-ABE9

    Plasmid
    #240625
    Purpose
    Adenine base editor with narrowed editing window, recognizing NG PAM
    Depositor
    Tingting Jiang
    Article
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Insert
    TadA8e (N108Q/L145T)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA8e (N108Q/L145T), Cas9 (D10A, L1111R, D1135V,…
    Available Since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240625-plasmid-map-sequence-id-479197

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  3. NG-ABE-NW

    Plasmid
    #240622
    Purpose
    Adenine base editor with narrowed editing window, recognizing NG PAM
    Depositor
    Tingting Jiang
    Article
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Insert
    TadA8e(N108Q, M151C, Q154Y)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA8e(N108Q, M151C, Q154Y), Cas9 (D10A, L1111R, …
    Available Since
    July 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240622-plasmid-map-sequence-id-480855

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  7. pSCBE3-NG

    Plasmid
    #218159
    Purpose
    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / L1111…
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218159-plasmid-map-sequence-id-433199

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  8. PBE-NG

    Plasmid
    #229045
    Purpose
    Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.
    Depositor
    Huawei Zhang
    Article
    Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    229045-plasmid-map-sequence-id-459812

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  20. PET28-FnCas12a-EP16

    Plasmid
    #183636
    Purpose
    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.
    Depositor
    Yunzi Luo
    Article
    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-
    Insert
    FnCas12a-EP16
    Use
    CRISPR
    Tags
    6×His Tag
    Expression
    Bacterial
    Mutation
    N607R, K613V, N617R, K180S, K660R, D616N
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183636-plasmid-map-sequence-id-375575

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  23. pGEX-2T-FRB-W2027F

    Plasmid
    #73341
    Purpose
    Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Jie Chen
    Article
    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; W2027F
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. pGEX-2T FRB Y2105A

    Plasmid
    #68726
    Purpose
    Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Jie Chen
    Article
    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; Y2105A
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  29. pIA028

    Plasmid
    #200419
    Purpose
    thrC::(PAmCherry1 erm2),for PAmCherry1 fusions.PALM integration vector for B. subtilis. Includes PAmCherry1 photoactivatable FP with multi cloning sites (MCS) for fast and interchangeable cloning
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016
    Type
    Empty backbone
    Tags
    PAmCherry1
    Expression
    Bacterial
    Available Since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    200419-plasmid-map-sequence-id-405382

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  33. Dat - EGFP - Tol2

    Plasmid
    #85418
    Purpose
    for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene
    Depositor
    Marc Ekker
    Article
    Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:
    Insert
    dopamine transporter (slc6a3 Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Promoter
    dat
    Available Since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 921 - 960 of 2180 results