We narrowed to 12,999 results for: LIC

1. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

   Plasmid

   #217798

   Purpose

   Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR and Lentiviral

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   EF1a

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

   Plasmid

   #217802

   Purpose

   Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)

   Plasmid

   #217806

   Purpose

   Unfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   mSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

   Plasmid

   #217796

   Purpose

   Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

   Plasmid

   #55220

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   His6 and MBP, Asn10

   Expression

   Insect

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

   Plasmid

   #55221

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   StrepII and msfGFP

   Expression

   Insect

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

   Plasmid

   #217808

   Purpose

   Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

   Plasmid

   #55222

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Available Since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

   Plasmid

   #55223

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Tags

   SNAPf and TwinStrepII

   Expression

   Insect

   Available Since

   July 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid

    #217797

    Purpose

    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid

    #217803

    Purpose

    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…

    Promoter

    CMV and T7

    Available Since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SPOCK2_22-424_AviN_HisC

    Plasmid

    #195877

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SPOCK2 (SPOCK2 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NXPH1_22-271_AviN_HisC

    Plasmid

    #195872

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NXPH1 (NXPH1 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SPOCK1_23-439_HisN_AviC

    Plasmid

    #195876

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SPOCK1 (SPOCK1 Human)
    

    Use

    Pfhmsp-avic-lic-n, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. OLFML3_123-406_AviN_HisC

    Plasmid

    #195871

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    OLFML3 (OLFML3 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. NTN1_39-453_AviN_HisC

    Plasmid

    #195873

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    NTN1 (NTN1 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. COL11A1_36-250_HisN_AviC

    Plasmid

    #195878

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    COL11A1 (COL11A1 Human)
    

    Use

    Pfhmsp-avic-lic-n, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. COL11A1_1570-1806_HisN_AviC

    Plasmid

    #195879

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    COL11A1 (COL11A1 Human)
    

    Use

    Pfhmsp-avic-lic-n, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ECE1_101-770_HisN_AviC

    Plasmid

    #195875

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ECE1 (ECE1 Human)
    

    Use

    Pfhmsp-avic-lic-n, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. COL25A1_53-654_AviN_HisC

    Plasmid

    #195880

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    COL25A1 (COL25A1 Human)
    

    Use

    Pfhmsp-avin-lic-c, baculovirus expression

    Promoter

    Polyhedrin

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge