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Showing: 461 - 480 of 593 results

  1. qTAG-C-mStayGold-EFS-Puro-PLK4

    Plasmid
    #227311
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227311-plasmid-map-sequence-id-452079

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  2. TFORF2737

    Plasmid
    #144154
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PLK4 (PLK4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144154-plasmid-map-sequence-id-396231

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  4. LentiV_Neo_LKB1_CR

    Plasmid
    #108112
    Purpose
    Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    LKB1 (STK11 Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    change cytosine 333 to alanine (silent mutation)
    Promoter
    EFS promoter
    Available since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108112-plasmid-map-sequence-id-207845

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  16. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

    Plasmid
    #69842
    Purpose
    This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag
    Depositor
    Michel Bornens
    Article
    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.
    Insert
    PLK4 (PLK4 Human)
    Use
    Tags
    3xFLAG tag and EGFP
    Expression
    Mammalian
    Mutation
    K41M-S285A-T289A
    Promoter
    CMV
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69842-plasmid-map-sequence-id-123881

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  18. nes714tk/lacZ

    Plasmid
    #47614
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Article
    Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.
    Insert
    nestin 2nd intron fragment (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Expression
    Mutation
    714 bp from 3' end of 2nd intron
    Promoter
    HSV tk promoter
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. nes1852tk/lacZ

    Plasmid
    #47613
    Purpose
    used to create transgenic mice expressing LacZ reporter with Nestin enhancer
    Depositor
    Urban Lendahl
    Article
    Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.
    Insert
    nestin 2nd intron (NES Human)
    Use
    Enhancer reporter
    Tags
    HSV tk promoter and lacZ
    Expression
    Mutation
    2nd intron only
    Promoter
    HSV tk promoter
    Available since
    Aug. 28, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 461 - 480 of 593 results