Addgene

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pCS2 stabilized mutant Beta Catenin-GFP

Plasmid

#29684

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

beta catenin (ctnnb1.L Frog)

Use

Tags

GFP

Expression

Mammalian

Mutation

S33A, S37A, T41A

Promoter

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-puro/RAF1-S259A

Plasmid

#131727

Purpose

Mammalian Expression of RAF1

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

RAF1 (RAF1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S259A

Promoter

CMV

Available since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2 deltaB

Plasmid

#16067

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Deleted Basic Domain (aa1-44)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
Nuclear Myosin I YFP

Plasmid

#60241

Purpose

Expresses nuclear isoform of myosin IC attached to YFP and an NLS

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-NMYC TRF2 deltaM

Plasmid

#16068

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Deleted Myb Domain (aa454-500)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaA deltaM

Plasmid

#16065

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted acidic domain (aa1-66) and myb domain (aa…

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaM

Plasmid

#16064

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted myb domain (aa379-439)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaA

Plasmid

#16063

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted acidic domain (aa1-66)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
AllCB shRNA-1

Plasmid

#160065

Purpose

Knock Down all Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA421-Cas9tr

Plasmid

#138709

Purpose

Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

Depositor

Víctor de Lorenzo

Article

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Insert

tracrRNA-Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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PCDHGC5-EGFP

Plasmid

#119933

Purpose

Protocadherin gamma C5 expression (EGFP-tag at the C-terminus).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Septin 11 isoform II

Plasmid

#120317

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-N FLAG

Plasmid

#12521

Depositor

Titia de Lange

Insert

N FLAG

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only

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mRFP-human APPL1

Plasmid

#22202

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Adaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)

Use

Tags

mRFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2 deltaB deltaM

Plasmid

#16069

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

Deleted Basic Domain (aa1-44) and Myb Domain (aa4…

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pENTR_Hu_PRC1(WT)

Plasmid

#136349

Purpose

Human PCR1 cDNA in Gateway pENTR

Depositor

Guillermo de Cárcer

Insert

PRC1 (PRC1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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E407V Nuclear Myosin I YFP

Plasmid

#60242

Purpose

Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Use

Tags

EYFP and NLS

Expression

Mammalian

Mutation

E407V

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCS2 hSmad1-EVE

Plasmid

#22993

Depositor

Edward De Robertis

Article

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Insert

Smad1 (SMAD1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S463E, S465E

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pCD5-H5_Vietnam_1203/04_sfGFP_ST

Plasmid

#182546

Purpose

Expresses a sfGFP fused H5 protein of the prototype A/Vietnam/1203/04 virus

Depositor

Robert de Vries

Article

Broszeit et al Cell Rep. 2019 Jun 11;27(11):3284-3294.e

Insert

H5_Vietnam_1203_04

Use

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP-TRF1 pWzl-Hygro

Plasmid

#19834

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Oct 19. ():.

Insert

Telomere-Repeat binding Factor 1 (Terf1 Mouse)

Use

Retroviral

Tags

enhanced GFP

Expression

Mutation

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only