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pRAJ21

Plasmid

#39249

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1+pLtetO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRAJ21m

Plasmid

#39250

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

RAJ21 system (no transRNA)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pHMMa.PH1704

Plasmid

#11452

Depositor

Sung-Hou Kim

Article

Du et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

Insert

intracellular protease

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
Phi80 JQ929581

Plasmid

#38170

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Integration vector

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only
hIRmut-GFP

Plasmid

#22397

Depositor

Joseph Bass

Article

Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

Insert

IRmut

Tags

GFP

Expression

Mammalian

Mutation

changed Leu 62 to Proline

Available Since

Nov. 29, 2011

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H1237

Plasmid

#64290

Purpose

OmpA redesign hybrid with wild-type strands 1237

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1237

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H-3

Plasmid

#64310

Purpose

OmpA redesign hybrid with wild-type strands 1245678

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H-3

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pT7ddx19gRNA

Plasmid

#47932

Purpose

in vitro transcription of ddx19 gRNA

Depositor

Wenbiao Chen, Susan Wente

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

ddx19 gRNA (ddx19 Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pSTC0r1

Plasmid

#39242

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Insert

pLlacO-1+BBa_B0034+GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pJexpress414_OmpA171_R4cons_G41A

Plasmid

#64299

Purpose

Conservative version of OmpA Redesign 4 with G41A mutation

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons_G41A

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_R4cons

Plasmid

#62639

Purpose

Conservative version of OmpA Redesign 4

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

25 mutations to lipid-facing surface

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
piHGpd/P82

Plasmid

#1325

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

GPD promoter-HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
K218Tc-3

Plasmid

#1300

Depositor

Susan Lindquist

Article

Schirmer et al Proc Natl Acad Sci U S A 2001 Jan 30;98(

Insert

mutant HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

K218T: A315T

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pSL012

Plasmid

#246580

Purpose

express mGreen Lantern from the cadBA promoter

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

mGreenLantern

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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PTGR-PS2

Plasmid

#237442

Purpose

Expressing PS2 S-layer from C. glutamicum under its native promoter

Depositor

Han Remaut

Article

Sogues et al Proc. Natl. Acad. Sci. U.S.A. 122 (31) e

Insert

cpsB

Expression

Bacterial

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCTcon2-dHUH

Plasmid

#235187

Purpose

To display dHUH on yeast surface

Depositor

Alice Ting

Article

Huang et al Proc Natl Acad Sci U S A. 2025 Mar 4;122

Insert

dHUH

Tags

HA, aga2p, and myc

Expression

Yeast

Available Since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

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pAM259

Plasmid

#226903

Purpose

mEOS3.2-MBP-Pex15(1-253)-PSP-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

mEOS3.2

Tags

mEOS3.2-MBP-Pex15(1-253)-PSP-FLAG-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM244

Plasmid

#226888

Purpose

6xHis-PSP-GS-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Tags

His-Precission

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Im7 WT

Plasmid

#201081

Purpose

Im7 WT inserted into CysGA at G364 via GS linker

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Im7

Expression

Bacterial

Promoter

pTrc

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pTrc-CysGA-Linker only

Plasmid

#201080

Purpose

GS linker inserted into CysGA at G364

Depositor

Shu Quan

Article

Ren et al Proc Natl Acad Sci U S A. 2021 Mar 30;11

Insert

CysGA-Linker only

Expression

Bacterial

Promoter

pTrc

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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