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pEAHISMRSA0482

Plasmid

#96968

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 0482

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

MRSA ORF0482

Tags

N-ter TEV protease cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRb delta CDK + S230

Plasmid

#58907

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

Depositor

Steven Dowdy

Publication

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S230 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T252

Plasmid

#58910

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

Depositor

Steven Dowdy

Publication

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T252 restored

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
RB1-TP53-LRG-GFP

Plasmid

#225876

Purpose

Guides targeting TP53 and RB1

Depositor

Janai Carr-Ascher

Publication

Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

Insert

RB1 and TP53 (TP53, RB1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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RB-409-815-pGex2T

Plasmid

#21784

Depositor

William Kaelin

Publication

Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 409-815 of wt Rb …

Available since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pHH0103_FGR-1/1

Plasmid

#91543

Purpose

Protein expression and purification of human SH3 domain construct FGR-1/1

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FGR-1/1 (FGR Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHL601

Plasmid

#37564

Depositor

Han Lim

Publication

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

CsrC (csrC E. coli)
CsrA (csrA E. coli)

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
Rb-379-928Δ703-737-pGex2T

Plasmid

#21933

Depositor

William Kaelin

Publication

Kaelin et al Cell. 1991 Feb 8. 64(3):521-32.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928D703-737 o…

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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FGR gRNA (BRDN0001146726)

Plasmid

#75719

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGR

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGR (FGR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FGR gRNA (BRDN0001147671)

Plasmid

#75720

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGR

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGR (FGR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FGR gRNA (BRDN0001147903)

Plasmid

#75721

Purpose

3rd generation lentiviral gRNA plasmid targeting human FGR

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FGR (FGR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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Rb379-928/C706F-pGex2TK

Plasmid

#21750

Depositor

William Kaelin

Publication

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928 of wt Rb …

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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pEANHISECArdB

Plasmid

#96967

Purpose

Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

E. coli ArdB

Tags

N-ter TEV cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.Y756C

Plasmid

#82863

Purpose

Gateway Donor vector containing RB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RB1 (RB1 Human)

Use

Gateway Cloning

Mutation

Y756C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2676

Plasmid

#96970

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 2676

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Sar2676

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAMVAK

Plasmid

#97000

Purpose

expression of methicillin-resistant Staphylococcus aureus MVAK protein

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Mevalonate kinase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPApqsE

Plasmid

#97019

Purpose

Expressing Pseudomonas aeruginosa pqsE protein

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Quinolone signal response protein

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pWzyE-distrepII

Plasmid

#196667

Purpose

Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Publication

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

TEV-cleavable di-strep tag II

Expression

Bacterial

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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PIGP_pCSdest

Plasmid

#53827

Depositor

Roger Reeves

Publication

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PIGP (PIGP Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
TtEnd5A_pEHISTEV

Plasmid

#179732

Purpose

TtEnd5A_pEHISTEV expression vector encodes first Thermogutta terrifontis Endoglucanase (19-531 amino acid residues) of Glycoside Hydrolase Family 5.

Depositor

James Naismith

Publication

Hussain et al Arch Biochem Biophys. 2025 Feb;764:11027

Insert

TtEnd5A

Tags

6xHis

Expression

Bacterial

Mutation

19 Amino acids from N-terminal encoding Signal pe…

Promoter

T7

Available since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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