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Plasmid#25227PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWDR5_01 (WDR5 Human)
UseTagsHisExpressionBacterialMutationlacking residues 1–23PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
EV-A71 isolate 4643-TW98 2A precursor protease with C110A and four aa at N-term to create a VP1-2A junction in active site
Plasmid#228633PurposeBacterial expression of the A71 2A protease with ITTL at the N-terminal and a C110A mutation used for crystallization in P 6 1 (PDB 9FGO)DepositorInsertEnterovirus Coxsackievirus A71 2A protease
UseTagsHis-SUMOExpressionBacterialMutationC110APromoterAvailable sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNoT7
Plasmid#218168PurposepET31b plasmid with short insert lacking T7 and ribosome binding sites as controlDepositorInsertShort sequence, mutated RBS and T7 sites
UseTagsExpressionBacterialMutationPromoterNA - mutated T7Available sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP Cav2.2 HA L702 P pCAGGS
Plasmid#206055Purposeexpression of rabbit Cav2.2 with N-terminal GFP tag and exofacial double HA tag in domain II and a L702P mutation that allows trafficking to the plasma membrane but channel is not functionalDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsGFPExpressionMammalianMutationL702PPromoterCMV/B-actinAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Atg32-deltaPsR pXP721
Plasmid#200735PurposePlasmid for the expression of GFP-Atg32 lacking the PsR domain in S. cerevisiae.DepositorInsertAtg32
UseTagsGFPExpressionYeastMutationAmino acids 200-341 are deleted.PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3DsR
Plasmid#182206Purpose3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescueDepositorInsertrTetR-3XP3-DsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
1xTRE DREaMR 3p3DsR
Plasmid#182204Purpose1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-DsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB237.1
Plasmid#170031PurposeEncodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL19 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 ftsY
Plasmid#117130PurposeFtsY gene cassetteDepositorInsertftsY
UseGolden gate donor vector for gene targeting in ba…TagsExpressionMutationPromoterAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pKB235.4
Plasmid#170026PurposeEncodes LacI-controlled expression of surface-displayed CCL20 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
CCL20 Δ alpha-helix
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-mCherry
Plasmid#65301Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-AP-Ub-dGG
Plasmid#232581PurposeExpress Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycinesDepositorInsertUbiquitin
UseTagsAviTagExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_VSVG-SBP-EGFP
Plasmid#65300Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHFS:SEP-rGluA2 TKIT donor
Plasmid#169440PurposeDonor DNA for TKIT knockin of SEP to GluA2 in ratDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluN2A TKIT donor
Plasmid#169429PurposeDonor DNA for TKIT knockin of SEP to NR2A in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluN2B TKIT donor
Plasmid#169431PurposeDonor DNA for TKIT knockin of SEP to NR2B in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluN1 TKIT donor
Plasmid#169427PurposeDonor DNA for TKIT knockin of SEP to NR1 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only