Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,759 results for: nls

Clear keywords
Showing: 9561 - 9600 of 18759 results

  4. PX458-3xHA-en31FnCas9ABEmax8.17d

    Plasmid
    #201956
    Purpose
    Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Publication
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Promoter
    Cbh
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201956-plasmid-map-sequence-id-405311

    Enlarge

  7. pRDB_053

    Plasmid
    #216083
    Purpose
    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    Use
    Destination
    Expression
    Insect
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216083-plasmid-map-sequence-id-431419

    Enlarge

  9. pAAV-CMV-SpCas9D10A nickase

    Plasmid
    #216737
    Purpose
    Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)
    Depositor
    Vincent Dion
    Publication
    Alvaro et al bioRxiv
    Insert
    Cas9-D10A nickase
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CMVd1
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    216737-plasmid-map-sequence-id-430337

    Enlarge

  11. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid
    #217304
    Purpose
    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter
    Depositor
    Luke Gilbert
    Insert
    dCas9-tagBFP-KRAB
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Promoter
    Ef1alpha
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    217304-plasmid-map-sequence-id-429717

    Enlarge

  13. AID-C12*-dCas9-BE4max

    Plasmid
    #216731
    Purpose
    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.
    Depositor
    Rahul Kohli
    Publication
    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-C12*-dCas9-BE4max (AICDA Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    216731-plasmid-map-sequence-id-429722

    Enlarge

  16. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216251
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus
    Depositor
    Elina Ikonen
    Publication
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9 and sgSEC61B.N1 (SEC61B Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216251-plasmid-map-sequence-id-426855

    Enlarge

  17. pMK33-GW-TurboID-V5

    Plasmid
    #196281
    Purpose
    Gateway destination vector to express C-terminal TurboID fusion under metallothionein promoter, for making stable cell lines
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway Cloning
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196281-plasmid-map-sequence-id-384554

    Enlarge

  19. pCH62 (denAsCas12a-KRAB piggyBac)

    Plasmid
    #217333
    Purpose
    piggyBac expression of denAsCas12a-KRAB
    Depositor
    Luke Gilbert
    Publication
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    denAsCas12a
    Use
    CRISPR
    Tags
    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2
    Expression
    Mammalian
    Mutation
    D908A/E174R/S542R/K548R
    Promoter
    CAG
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217333-plasmid-map-sequence-id-426481

    Enlarge

  20. pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry

    Plasmid
    #214470
    Purpose
    Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    ZFYVE27 (ZFYVE27 Human)
    mCherry
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214470-plasmid-map-sequence-id-425102

    Enlarge

  24. P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

    Plasmid
    #202762
    Purpose
    Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor
    Depositor
    Karl Wahlin
    Publication
    Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NEUROG2 (NEUROG2 Human)
    Expression
    Mammalian
    Promoter
    TetO
    Available since
    Feb. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    202762-plasmid-map-sequence-id-416794

    Enlarge

  25. pPB-spCas9-pNeo

    Plasmid
    #214120
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Available since
    Feb. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    214120-plasmid-map-sequence-id-423617

    Enlarge

  27. pCMV-RR-ABE8e-V106W

    Plasmid
    #193656
    Purpose
    Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-TadA*8e-V106W, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K595R, TadA*8e-V106W
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193656-plasmid-map-sequence-id-422133

    Enlarge

  28. pCMV-dCas12a-ABE7

    Plasmid
    #193644
    Purpose
    Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-TadA-TadA*7.10, EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193644-plasmid-map-sequence-id-421946

    Enlarge

  29. LbCas12a-nuCPE

    Plasmid
    #213748
    Purpose
    For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCP-LbCas12a-M-MLV RT∆RNase H
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    D156R in LbCas12a
    Promoter
    CMV
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213748-plasmid-map-sequence-id-422187

    Enlarge

  35. pISV-CRISPR/Cas9PGmUbi

    Plasmid
    #209189
    Purpose
    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection
    Depositor
    Christian Staehelin
    Inserts
    Cas9
    DsRed(deltaB)
    Tags
    NLS
    Expression
    Plant
    Mutation
    missing BsaI site and plant-codon optimized with …
    Promoter
    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209189-plasmid-map-sequence-id-419145

    Enlarge

  36. pLV-SFFV-mCherry-WPRE-UbC-MCS

    Plasmid
    #211801
    Purpose
    Lentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    mCherry
    multiple cloning site
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211801-plasmid-map-sequence-id-418105

    Enlarge

  37. pLV-SFFV-MCS-WPRE-UbC-Emerald

    Plasmid
    #211796
    Purpose
    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Inserts
    multiple cloning site
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211796-plasmid-map-sequence-id-418059

    Enlarge

  38. ABE-RA3.0

    Plasmid
    #208301
    Purpose
    Expresses ABE-RA3.0 in mammalian cells
    Depositor
    Weixin Tang
    Publication
    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-RA3.0-SpCas9 D10A
    Expression
    Mammalian
    Mutation
    E27D, P48A, R51H, I76F, D108G, K110R, H122R, M126…
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    208301-plasmid-map-sequence-id-410368

    Enlarge

  39. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Sahand Jamal Rahi
    Publication
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211765-plasmid-map-sequence-id-417945

    Enlarge

Showing: 9561 - 9600 of 18759 results