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We narrowed to 52,941 results for: plasmid

Showing: 11951 - 12000 of 52941 results

  1. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177433
    Purpose
    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177433-plasmid-map-sequence-id-348464

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  3. ATF4 2: Mouse ATF4 (CHOP11/cATF)-bZIP

    Plasmid
    #21849
    Depositor
    David Ron
    Insert
    ATF4 (Atf4 Mouse)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    N-terminal transactivation domain deleted and rep…
    Promoter
    Available since
    Sept. 25, 2009
    Availability
    Academic Institutions and Nonprofits only
    21849-plasmid-map-sequence-id-10036

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  4. PANK1

    Plasmid
    #32871
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK1 (PANK1 Human)
    Use
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 231-597 only
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pIRESneo3+mRFP-Utr_CH

    Plasmid
    #64360
    Purpose
    bicistronic expression of mRFP-Utrophin and Neo resistance
    Depositor
    Matthieu Piel
    Insert
    mRFP-Utrophin (UTRN Human)
    Use
    Tags
    IRES-Neo and mRFP
    Expression
    Mammalian
    Mutation
    Q236R; aa1-261 Utrophin present
    Promoter
    CMV
    Available since
    May 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    64360-plasmid-map-sequence-id-196886

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  7. pCMV6-XL4 FLAG-CBLN2-Fc

    Plasmid
    #115767
    Purpose
    Expression of the extracellular domain of CBLN2 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    CBLN2 (CBLN2 Human)
    Use
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115767-plasmid-map-sequence-id-223830

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  8. CAG-loxP-3xpA-loxP-tdTomato+RbG and HsbG syntrons

    Plasmid
    #172479
    Purpose
    tdTomato with synthetic introns derived from the rabbit beta-globin gene and the human beta-globin gene
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172479-plasmid-map-sequence-id-338913

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  9. HARS_pNIC-Bio3

    Plasmid
    #153050
    Purpose
    Expression construct for producing Avi tagged histidyl tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    HARS (HARS1 Human)
    Use
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153050-plasmid-map-sequence-id-304465

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  10. pLNHA-C1-HsNot1iso1-shRNAres_V

    Plasmid
    #147749
    Purpose
    Mammalian Expression of HsNot1iso1-shRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsNot1iso1-shRNAres (CNOT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    1 silent mutation compared to the sequence of iso…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147749-plasmid-map-sequence-id-444359

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  11. pSpot-Tag-Actin

    Plasmid
    #117060
    Purpose
    For mammalian expression of beta-actin with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    actin beta (ACTB Human)
    Use
    Tags
    Spot-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117060-plasmid-map-sequence-id-224388

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  12. SMYD3

    Plasmid
    #32048
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SMYD3 (SMYD3 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pKG187

    Plasmid
    #63077
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FAAP24, IMAGE clone 3659284 (FAAP24 Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    63077-plasmid-map-sequence-id-134680

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  14. pcDNA5/FRT/TO SBBP-CDK1 WT (2007)

    Plasmid
    #61847
    Purpose
    mammalian expression of CDK1 WT, used for Ips
    Depositor
    Jonathon Pines
    Insert
    CDK1 (CDK1 Human)
    Use
    Tags
    SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. SPIN2B (SPIN2BA-c011)

    Plasmid
    #98238
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPIN2BA (SPIN2B Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98238-plasmid-map-sequence-id-193417

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  16. pcDNA3.1_hSMAD4

    Plasmid
    #212710
    Purpose
    The ORF of human SMAD4, variant 1 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    Homo sapiens SMAD4 (SMAD4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    212710-plasmid-map-sequence-id-448252

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  17. pAc5.1B-lambdaN-HA-DmARC1_N

    Plasmid
    #146978
    Purpose
    Insect Expression of DmARC1
    Depositor
    Elisa Izaurralde
    Insert
    DmARC1 (Arc1 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    5 silent mutations and one non silent mutation G3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146978-plasmid-map-sequence-id-389936

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  18. IDG_GLRA3_OE_1

    Plasmid
    #161677
    Purpose
    Overexpresses GLRA3
    Depositor
    Mike McManus
    Insert
    GLRA3 (GLRA3 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    161677-plasmid-map-sequence-id-319660

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  21. pIVTR(T7A)-ATOH1 (SA Substitutions)

    Plasmid
    #172309
    Purpose
    In vitro transcription of ATOH1 (Ser phosphosites substituted by Ala)
    Depositor
    Patrik Verstreken
    Insert
    ATOH1 (ATOH1 Human)
    Use
    Other
    Tags
    Expression
    Mutation
    S331A, S342A
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172309-plasmid-map-sequence-id-340456

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  22. pLp6.3-EglN3-H196A

    Plasmid
    #79116
    Purpose
    Lentiviral expression of EglN3-H196A
    Depositor
    Susanne Schlisio
    Insert
    EglN3 catalytic dead mutant (EGLN3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    catalytic dead mutant: H196A
    Promoter
    CMV
    Available since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79116-plasmid-map-sequence-id-149803

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  23. 4P7A

    Plasmid
    #61954
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    Use
    Tags
    6xHis and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    March 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pcDNA-zeo-hFJX1-myc

    Plasmid
    #46930
    Purpose
    expression of human FJX1-myc in mammalian cells
    Depositor
    Natasha Deane, R.D. Beauchamp
    Insert
    FJX1 (FJX1 Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. PRDM11

    Plasmid
    #32858
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM11 (PRDM11 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 79-314
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pLNCX1 Myr AKT2

    Plasmid
    #27294
    Depositor
    Morris Birnbaum
    Insert
    protein kinase B beta (AKT2 Human)
    Use
    Retroviral
    Tags
    HA and myristolation sequence
    Expression
    Mutation
    PH domain deleted (aa1-107)
    Promoter
    Available since
    April 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  27. pSpot-Tag-Lamin

    Plasmid
    #117062
    Purpose
    For mammalian expression of lamin B1 with N-terminal Spot-Tag
    Depositor
    Felix Hartlepp
    Insert
    Lamin B1 (LMNB1 Human)
    Use
    Tags
    Spot-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117062-plasmid-map-sequence-id-224387

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  29. pIC84

    Plasmid
    #63062
    Purpose
    Retroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    hCENP-H, IMAGE clone 3451767 (CENPH Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    63062-plasmid-map-sequence-id-104377

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  30. IDG_GABRG1_OE_1

    Plasmid
    #161673
    Purpose
    Overexpresses GABRG1
    Depositor
    Mike McManus
    Insert
    GABRG1 (GABRG1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    161673-plasmid-map-sequence-id-319665

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  31. pFETCh_ATF4

    Plasmid
    #72350
    Purpose
    Donor vector for 3' FLAG tag of human ATF4
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    ATF4 Homology Arms (ATF4 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Expression
    Mutation
    Promoter
    Available since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72350-plasmid-map-sequence-id-147903

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  32. pKG402

    Plasmid
    #69760
    Purpose
    Retroviral expression of CENP-T aa 256-561
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 256-561 (CENPT Human)
    Use
    Retroviral
    Tags
    mCherry-TEV-S
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  33. pCI_mini-mAgrin-AviTag

    Plasmid
    #198131
    Purpose
    Express mini-Agrin in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    mini-Agrin (Agrn Mouse)
    Use
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    198131-plasmid-map-sequence-id-393463

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  34. pKG210

    Plasmid
    #69761
    Purpose
    Retroviral expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  36. Vimentin/pAS2-1

    Plasmid
    #98136
    Purpose
    Vimentin (NM_003380) in DNA-binding domain (BD) vector, used for yeast-two hybrid
    Depositor
    Rita Shiang
    Insert
    Vimentin (VIM Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98136-plasmid-map-sequence-id-191128

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  37. IDG_KCNS3_OE_1

    Plasmid
    #161618
    Purpose
    Overexpresses KCNS3
    Depositor
    Mike McManus
    Insert
    KCNS3 (KCNS3 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    161618-plasmid-map-sequence-id-318723

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  38. pKG186

    Plasmid
    #63076
    Purpose
    Retroviral expression of EGFP-TEV-S-tag FANC-A for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    FANC-A, IMAGE clone 5725803 (FANCA Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    63076-plasmid-map-sequence-id-104390

    Enlarge

  39. pCMV6-XL4 FLAG-CBLN4-Fc

    Plasmid
    #115769
    Purpose
    Expression of the extracellular domain of CBLN4 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    CBLN4 (CBLN4 Human)
    Use
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115769-plasmid-map-sequence-id-223827

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  40. XLone-BSD NFIL3 P2A eGFP NLS

    Plasmid
    #140030
    Purpose
    Tunable and temporal expression control of NFIL3 and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    NFIL3 (NFIL3 Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140030-plasmid-map-sequence-id-275866

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  41. pLKO-Tet-puro-hARAF-shRNA-1

    Plasmid
    #185370
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF
    Depositor
    Ayaz Najafov
    Insert
    ARAF (ARAF Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    185370-plasmid-map-sequence-id-363837

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  43. CBS

    Plasmid
    #73238
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CBS (CBS Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    73238-plasmid-map-sequence-id-150431

    Enlarge

  44. p2Bac.V5.p62

    Plasmid
    #39218
    Depositor
    Aziz Sancar
    Insert
    p62 (GTF2H1 Human)
    Use
    Tags
    V5
    Expression
    Insect
    Mutation
    Promoter
    Pp10
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  45. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  47. p2Bac.Flag.p34

    Plasmid
    #39221
    Depositor
    Aziz Sancar
    Insert
    p34 (GTF2H3 Human)
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    Pph
    Available since
    Aug. 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  48. IDG_KCNG3_OE_1

    Plasmid
    #161644
    Purpose
    Overexpresses KCNG3
    Depositor
    Mike McManus
    Insert
    KCNG3 (KCNG3 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    161644-plasmid-map-sequence-id-319582

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  49. pE-SUMO CRYBB3

    Plasmid
    #80750
    Purpose
    Expression of human beta B3-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYBB3 (CRYBB3 Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80750-plasmid-map-sequence-id-152180

    Enlarge

  50. TTN/pBSSK+

    Plasmid
    #98124
    Purpose
    titin (NM_133378, nt 99776 to 100510) in pBSSK+
    Depositor
    Rita Shiang
    Insert
    TTN (TTN Human)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    98124-plasmid-map-sequence-id-191011

    Enlarge

Showing: 11951 - 12000 of 52941 results