We narrowed to 23,163 results for: CRISPR

1. pYPQ-dpcoCas9-Act3.0

   Plasmid

   #158408

   Purpose

   CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

   Plasmid

   #168107

   Purpose

   PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   Cas9-P2A-mCherry
   

   Use

   CRISPR and Cre/Lox

   Tags

   HA

   Promoter

   CAG

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_831

   Plasmid

   #216100

   Purpose

   CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB12A

   Plasmid

   #210035

   Purpose

   Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas12a
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

   Plasmid

   #140000

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. G1397 DddAtox-C–SaKKH-Cas9(D10A)

   Plasmid

   #157840

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   CRISPR

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-U6sgbbBsmbI-puro-2A-Fluc

   Plasmid

   #100277

   Purpose

   Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. dpYPQ230-D156R-ABE4

   Plasmid

   #195370

   Purpose

   dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   ecTadA8e-(GGGGS)x6-dLbCas12a-D156R
   

   Use

   CRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…

   Tags

   5' and 3' NLS

   Expression

   Plant

   Promoter

   ZmUBI1

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. dpYPQ230-D156R-CBE1

   Plasmid

   #195367

   Purpose

   dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
   

   Use

   CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

   Tags

   3xflag

   Expression

   Plant

   Promoter

   ZmUBI1

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. B52 + SMARCAL1 sgSTOP

    Plasmid

    #100715

    Purpose

    B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SMARCAL1 (cloned using BbsI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. B52 + SPRTN sgSTOP

    Plasmid

    #100709

    Purpose

    B52 plasmid expressing SPRTN sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SPRTN (cloned using BbsI)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid

    #104438

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    UBQ10

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA1_ASCL1

    Plasmid

    #64129

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF005-sgRNA-shuffle-in

    Plasmid

    #104441

    Purpose

    Provide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Insert

    sgRNA-transRNA transcription by U6 promoter
    

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDR366 RNF2

    Plasmid

    #47509

    Purpose

    human gRNA expression vector targeting RNF2

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-RNF2 (RNF2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgRNA2_ASCL1

    Plasmid

    #64130

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL2 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pVC299 VEGF Site#2

    Plasmid

    #47506

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 2 (VEGFA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid

    #171089

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3X FLAG and Nuclear Localization Signal

    Mutation

    D10A, H840A

    Promoter

    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. sgRNA4_ASCL1

    Plasmid

    #64132

    Purpose

    Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_ASCL4 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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