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pcDNA4/TO-BcRF1-ORF24-2xStrep

Plasmid

#138454

Purpose

Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

BcRF1-ORF24 (BcRF1, ORF24 EBV/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

BcRF1 amino acids 1-178 fused to ORF24 amino acid…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-UL87-ORF24-2xStrep

Plasmid

#138455

Purpose

Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87-ORF24 (UL87, ORF24 HCMV/KSHV)

Tags

Strep

Expression

Mammalian

Mutation

UL87 amino acids 1-248 fused to ORF24 amino acids…

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-F307Y-pKK223

Plasmid

#131381

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307Y in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera F307Y

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are from…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-F307A-pKK223

Plasmid

#131382

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change F307A in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera F307A

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are from…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-S308V-pKK223

Plasmid

#131393

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308V in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera S308V

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are fro…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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EcNGsC-S308A-pKK223

Plasmid

#131394

Purpose

Low level expression of E.coli G.stearothermophillus MutY chimera protein with amino acid change S308A in Gs MutY.

Depositor

Martin Horvath

Article

Russelburg et al ACS Chem Biol. 2019 Dec 27. doi: 10.1021

Insert

EcNGsC MutY chimera S308A

Expression

Bacterial

Mutation

Fusion of two MutY genes. Residues 1-225 are fro…

Promoter

tac

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 NPm

Plasmid

#126593

Purpose

Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of amino acids 1-65 and L329A/L331A in t…

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2 CCm

Plasmid

#126594

Purpose

Expresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian cells, Dox inducible in TetR cell lines

Depositor

Joanna Morris

Article

Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

Insert

SENP2 (SENP2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of amino acids 203-228 and siRNA resista…

Promoter

CMV

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a-3NLS-Klf4 TALE-GCN4

Plasmid

#120546

Purpose

Express 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 gene

Depositor

Yu Wang

Article

Zhao et al ACS Chem Biol. 2018 Mar 16;13(3):609-617

Insert

NLS, Klf4 TALE, GCN4 (KLF4 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pSlick-Neo-KANK1

Plasmid

#121983

Purpose

Tetracycline-inducible expression of untagged full-length KANK1

Depositor

GuangJun Zhang

Article

Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

Insert

KANK1 (KANK1 Human)

Use

Lentiviral

Promoter

Tight TRE promoter

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.2.mKO2

Plasmid

#85210

Purpose

TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCDX2 ObLiGaRe Donor vector/EPB64

Plasmid

#90017

Purpose

Donor vector for ObLiGaRe/ZFN mediated targeting to CDX2 exon1 locus

Depositor

Eric-Paul Bennett

Article

Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

Insert

MCS flanked by inverted CDX2 ZFN binding sites (CDX2 Human)

Expression

Bacterial

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC-mTRF1deltaBLM

Plasmid

#64163

Purpose

Retroviral vector expressing mouse TRF1 lacking BLM-binding motifs with N-terminal Myc tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

mTRF1 (Terf1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted amino acids 313-316 and 339-342 (both BLM…

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Article

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mRuby2-CCR5TC

Plasmid

#135531

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FuG-E

Plasmid

#67509

Purpose

This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.

Depositor

Kazuto Kobayashi

Article

Kato et al J Neurosci Methods. 2014 Apr 30;227:151-

Insert

Fusion Glycoprotein type E

Use

Lentiviral

Tags

Fusion protein of Vesicular stomatitis Indiana vi…

Promoter

CAGGS

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph15-mNeonGreen-CCR5TC

Plasmid

#135533

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to mNeonGreen, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Tags

mNeonGreen

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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V5-ERG

Plasmid

#118623

Purpose

Expresses V5 tagged ERG protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (ERG Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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