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pGMEH-0X750-luxAB-DAS+4

Plasmid

#49519

Purpose

Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; replicates episomally

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

P750-luxAB-DAS+4

Tags

DAS+4 (AANDENYSENYADAS)

Expression

Bacterial

Promoter

P750

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pET41a iGlum

Plasmid

#119829

Purpose

Production of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGlum

Tags

GST

Expression

Bacterial

Mutation

R24K

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 dE5

Plasmid

#169263

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 delta E5 (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted region corresponding to human Exon 5

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 dE8

Plasmid

#169264

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 delta E8 (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted region corresponding to human Exon 8

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE5

Plasmid

#169267

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E5 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 5

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 dE8

Plasmid

#169268

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 delta E8 (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

deleted Exon 8

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E8 G>T minigene

Plasmid

#169272

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 8 G>T minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E8 G>T minigene (RBM28 Human)

Expression

Mammalian

Mutation

NM_018077.2:c.(946G>T)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 Exon1-6 E5 WT minigene

Plasmid

#169273

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 1-6 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 Exon1-6 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 Exon1-6 E5 GT>A minigene

Plasmid

#169274

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 1-6 GT>A minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 Exon1-6 GT>A minigene (RBM28 Human)

Expression

Mammalian

Mutation

NM_018077.2:c.(541+1_541+2delinsA)

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 E321G

Plasmid

#40981

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

E321G

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
CRISPRi sgSOS1-2

Plasmid

#253093

Purpose

CRISPRi single-guide against SOS1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

SOS1 (SOS1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgNF1-1

Plasmid

#253096

Purpose

CRISPRi single-guide against NF1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

NF1 (NF1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgSOS1-1

Plasmid

#253092

Purpose

CRISPRi single-guide against SOS1

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

SOS1 (SOS1 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CRISPRi sgPTPN11-1

Plasmid

#253090

Purpose

CRISPRi single-guide against PTPN11

Depositor

Harish Vasudevan

Article

Vasudevan et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

PTPN11 (PTPN11 Human)

Use

CRISPR

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-human SLC9C1-3xFlag

Plasmid

#249411

Purpose

Expression vector of human SLC9C1 tagged with 3xFLAG at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

SLC9C1 (SLC9C1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Tmem217-3xFlag

Plasmid

#249409

Purpose

Expression vector of mouse Tmem217 tagged with 3xFLAG at C-terminus under the CAG promoter.

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Transmembrane protein 217 (Tmem217 Mouse)

Expression

Mammalian

Available Since

Feb. 25, 2026

Availability

Academic Institutions and Nonprofits only

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pCAG-mouse Slc9c1-PA

Plasmid

#249412

Purpose

Expression vector of mouse SLC9C1 tagged with PA at C-terminus under the CAG promoter

Depositor

Masahito Ikawa

Article

Iida-Norita et al Proc Natl Acad Sci U S A. 2025 Oct 21;12

Insert

Slc9c1 (Slc9c1 Mouse)

Tags

PA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(K55R)-Avi

Plasmid

#245186

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

K55R

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-SHP2-N-SH2(T52S)-Avi

Plasmid

#245185

Purpose

Production of N-terminally His-SUMO-tagged and C-terminally Avi-tagged SH2 domain

Depositor

Neel Shah

Article

van Vlimmeren et al Proc Natl Acad Sci U S A. 2024 Jul 23;12

Insert

SHP2 N-SH2

Tags

Avi and His-SUMO

Expression

Bacterial

Mutation

T52S

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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