We narrowed to 59,445 results for: cloning

1. pJAT77

   Plasmid

   #204295

   Purpose

   pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-D-Topo-hTRIM9

   Plasmid

   #51032

   Purpose

   entry vector for cloning human TRIM9

   Depositor

   Timothy Cox

   Article

   Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

   Insert

   TRIM9 (TRIM9 Human)
   

   Use

   Entry vector

   Promoter

   NA

   Available Since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AbVec2.1-IGLC2-MscI

   Plasmid

   #80797

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, human lambda 2 constant region

   Depositor

   Hedda Wardemann

   Article

   Tiller et al J Immunol Methods. 2009 Oct 31;350(1-2):

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX1

   Plasmid

   #46848

   Purpose

   Vector modified from pBluescript SK(+) by adding more cloning sites

   Depositor

   Monica Hollstein

   Article

   Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK-N. tabacum atpB/accD

   Plasmid

   #123582

   Purpose

   MoChlo kit

   Depositor

   Scott Lenaghan, C. Neal Stewart

   Article

   Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

   Insert

   N. tabacum atpB/accD integration site
   

   Use

   Chloroplast transformation vector

   Expression

   Plant

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LRG2.1-CyOFP1

   Plasmid

   #124771

   Purpose

   3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGZ003

   Plasmid

   #48869

   Purpose

   Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Type

   Empty backbone

   Use

   Golden gate compatible plant transformation vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGGZ001

   Plasmid

   #48868

   Purpose

   Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Type

   Empty backbone

   Use

   Golden gate compatible plant transformation vector

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC18T-mini-Tn7T

   Plasmid

   #64957

   Purpose

   mini-Tn7 base vector with transcriptional terminator and oriT

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pFCM1

    Plasmid

    #64948

    Purpose

    source of CmR FRT cassette

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    FRT-cat-FRT
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. LRG2.1-mOrange

    Plasmid

    #124772

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MXS_CreERT2

    Plasmid

    #62427

    Purpose

    MXS_chaining vector with CreERT2

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    Tamoxifen inducible Cre recombinase
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAMP-CY1_NF1 T1 (+minintr)

    Plasmid

    #119204

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 1 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-tdTomato no stop

    Plasmid

    #82404

    Purpose

    Gateway-compatible tdTomato fusion reporter

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    tdTomato (lacks stop codon)
    

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGM1

    Plasmid

    #64949

    Purpose

    source of GmR FRT cassette

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    FRT-GmR-FRT
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human NCKX6 (pcDNA3.1+)

    Plasmid

    #75216

    Purpose

    Mammalian expression of SLC8B1

    Depositor

    Jonathan Lytton

    Article

    Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

    Insert

    SLC8B1 (SLC8B1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDD363

    Plasmid

    #91829

    Purpose

    LoxP-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    LoxP-SEC-LoxP
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV UBC APEX2-3xFLAG-IRES-eGFP

    Plasmid

    #214983

    Purpose

    Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    APEX2 (APX2 Glycine max (soybean))
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Promoter

    UBC

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_NKX2-5

    Plasmid

    #221479

    Purpose

    Gateway cloning of NKX2-5 overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-5 (NKX2-5 Human)
    

    Use

    Gateway donor vector

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFH35

    Plasmid

    #126009

    Purpose

    Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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