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pGEMHE-mClover3-KASH5-DN

Plasmid

#171492

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Publication

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-GST-KASH5-DN

Plasmid

#171493

Purpose

T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

Depositor

Melina Schuh

Publication

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

KASH5-DN (CCDC155 B. tarus (bovine))

Use

Pgehme

Tags

GST

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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C234-E26: attB2r-Halotag-attB3

Plasmid

#162915

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal Halotag fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Halotag

Use

Synthetic Biology

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E11: eGFP

Plasmid

#162893

Purpose

Standard Gateway entry clone encoding eGFP fluorescent protein

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

enhanced Green fluorescent protein

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E53: mClover3

Plasmid

#162898

Purpose

Standard Gateway entry clone encoding mClover fluorescent protein

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Clover3 fluorescent protein

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDH42(CUG/A)

Plasmid

#154132

Purpose

Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Publication

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

engineered bacterial rRNAs operon under control of pL promoter

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSM10

Plasmid

#139034

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mScarlet (no STOP codon)

Expression

Worm

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH60

Plasmid

#139029

Purpose

MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

spe-11 promoter (spe-11 Nematode)

Expression

Worm

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcF-1

Plasmid

#131023

Purpose

Single spacer array with spacer #1 for FnCas12a.

Depositor

Chase Beisel

Publication

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG050

Plasmid

#113999

Purpose

SC module. Thermostable beta-galactosidase reporter

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

celB

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOGG083

Plasmid

#114001

Purpose

SC module. Beta-glucosidase reporter

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

gusA

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTK473

Plasmid

#114715

Purpose

sgRNA for LGN's N-terminal locus

Depositor

Tomomi Kiyomitsu

Publication

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LGN sgRNA

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Bottom-T

Plasmid

#87310

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pWT015a

Plasmid

#96878

Purpose

GFP reporter

Depositor

David Liu

Publication

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP

Expression

Mammalian

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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YCe1891 HC_Kan_SV40-ORI_p25

Plasmid

#100679

Purpose

part designed to occupy position 25 of EMMA. Functional category: Viral origin of replication

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

SV40-ORI

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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LI D-E c-myc

Plasmid

#54451

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

c-MYC epitope tag (EQKLISEEDL)

Use

Cloning vector

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C c-myc

Plasmid

#54450

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

c-MYC epitope tag (EQKLISEEDL)

Use

Cloning vector

Available since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI D-E mCherry + link c1

Plasmid

#54420

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + mCherry

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMRS156.2 (miR156 sensor)

Plasmid

#27412

Depositor

David Bartel

Publication

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

miR156 target site

Tags

GFP

Available since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only

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ZE2 Wnt-4

Plasmid

#17043

Depositor

Randall Moon

Publication

Ungar et al Mech Dev. 1995 Aug;52(2-3):153-64. doi:

Insert

wnt-4 cDNA (wnt4a Zebrafish)

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only