We narrowed to 13,238 results for: BASE
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Plasmid#105864PurposeBacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1DepositorInsertK-GECO1
Tags6-His TagExpressionBacterialAvailable SinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas9-mD3A
Plasmid#78257PurposeExpresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoterDepositorInsertDNMT3A CD aa 598-912 (DNMT3A Human)
UseCRISPRTagsFlag epitope tagExpressionMammalianMutationE756APromoterpCMVAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En01
Plasmid#71534PurposeUsed to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
m.3047-Left TALE-G1397-N-dead-mCherry
Plasmid#211339PurposeExpression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selectionDepositorInsertm.3047-Left TALE-G1397-N-dead-mCherry
UseTALENExpressionMammalianPromoterCMVAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.3075-Left TALE-G1397-N-dead-mCherry
Plasmid#211340PurposeExpression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selectionDepositorInsertm.3075-Left TALE-G1397-N-dead-mCherry
UseTALENExpressionMammalianPromoterCMVAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
m.5147-Left TALE-G1397-N-dead-mCherry
Plasmid#211341PurposeExpression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selectionDepositorInsertm.5147-Left TALE-G1397-N-dead-mCherry
UseTALENExpressionMammalianPromoterCMVAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE.puro/TO/Flag/HuR.S221D
Plasmid#110347PurposeDoxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG tagDepositorInsertELAV like RNA binding protein 1 (ELAVL1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationS221D, P237SPromotergagAvailable SinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE.zeo/hKGA.S286A
Plasmid#110357PurposeMammalian retroviral expression of glutaminase isoform KGA (S286A) with V5 tagDepositorInsertKGA glutaminase (GLS Human)
UseRetroviralTagsV5ExpressionMammalianMutationS286APromotergagAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Nuclease-GFP
Plasmid#171994PurposeDelivers all prime editing nuclease components in a single, GFP selectable plasmidDepositorInsertCbH-Cas9-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
ExpressionMammalianMutationGC to CA point mutation in SpCas9 to restore nucl…PromoterCMV for Cas9, U6 for gRNAsAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-tev-std
Plasmid#109423PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope
TagsBAP, HA, His6, and T7ExpressionBacterialPromoterT7Available SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_GAL4UAS-Luciferase reporter
Plasmid#64157PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-epegRNA-SV40-puro
Plasmid#240539PurposeLentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.DepositorInsertepegRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEHAC1-HA-BirA-GSGS-Traf6
Plasmid#232586PurposeExpress BirA tagged to the N-terminus of TRAF6 with a GSGS linker between BirA and TRAF6.DepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEHAC1-CHIP-GSGS-BirA-HA
Plasmid#232588PurposeExpress BirA tagged to the C-terminus of CHIP with a GSGS linker between BirA and CHIP.DepositorAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMSCVhyg
Plasmid#134323PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
B-GRAM-H
Plasmid#211706PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cellsDepositorInsertB-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
TagsddFP-B3ExpressionMammalianMutationChanged Glycine 187 to LeucinePromoterCMVAvailable SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAIP-hXRCC1wt-eGFP
Plasmid#206032PurposeMammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoterDepositorInserthXRCC1
TagseGFPExpressionMammalianPromoterCAGAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)
Plasmid#211767PurposedCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selectionDepositorInsertdCas9, Spy, Snoop, aGCN4, AviTags
Tags3xHAExpressionMammalianMutationdCas9 D10A and H840APromoterpGK and pEF1aAvailable SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA13-GG
Plasmid#208002Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
Promoter14GAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS
Plasmid#199346PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only