We narrowed to 10,454 results for: ESP

1. pRevTRE MKL1

   Plasmid

   #19846

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

2. p3xFLAG-MKL1

   Plasmid

   #11978

   Purpose

   For mammalian expression of human MKL1 with a 3x FLAG tag

   Depositor

   Ron Prywes

   Article

   Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

   Insert

   MKL1 (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N579S T716A

   Promoter

   CMV

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMKO.1 puro hTERT shRNA

   Plasmid

   #10688

   Depositor

   William Hahn

   Article

   Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

   Insert

   hTERT shRNA (TERT Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-tight-DDdCas9GFP-IRES-Neo

   Plasmid

   #102907

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET151-GyrA

   Plasmid

   #216229

   Purpose

   S. aureus USA300 GyrA Codon-optimized for E. coli

   Depositor

   Eric Brown

   Article

   French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

   Insert

   DNA gyrase
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA_RGG-GFP-RGG

   Plasmid

   #124930

   Purpose

   Expression of RGG-GFP-RGG

   Depositor

   Matthew Good, Daniel Hammer, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI SMN1

   Plasmid

   #72286

   Purpose

   SMN1 exon 7 splicing cassette

   Depositor

   Elliot Androphy

   Article

   Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

   Insert

   SMN1 (SMN1 Human)
   

   Expression

   Mammalian

   Mutation

   exons 6-7-8 including introns

   Promoter

   CMV

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pETDuet RagA-RagC

   Plasmid

   #99641

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAT005

   Plasmid

   #171632

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mNeon
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1-a and U6

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4046887

    Plasmid

    #145730

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pET_RGG-RGG

    Plasmid

    #124941

    Purpose

    Expresion of RGG-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKDsgRNA-rssA

    Plasmid

    #89957

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rssA gRNA
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDV24_AAV2_DART-VADAR_iRFP720-sensor

    Plasmid

    #200411

    Purpose

    AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    DART VADAR sensor
    

    Use

    AAV and Synthetic Biology

    Tags

    MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-15b PAI-RBP

    Plasmid

    #45390

    Depositor

    Thomas Gelehrter

    Article

    Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

    Insert

    PAI-RBP (SERBP1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 20, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-EF1a-DIO-tdTomato-WPRE

    Plasmid

    #133786

    Purpose

    Cre-dependent tdTomato fluorescent reporter for cell labeling

    Depositor

    Michael Stryker

    Article

    Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

    Insert

    pAAV-EF1a-DIO-tdTomato-WPRE
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG68 G6PD 3'UTR

    Plasmid

    #12020

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    G6PD 3'UTR (G6PD Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET151-GyrB

    Plasmid

    #216233

    Purpose

    S. aureus USA300 GyrB Codon-optimized for E. coli

    Depositor

    Eric Brown

    Article

    French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

    Insert

    DNA gyrase
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TRE-DIO-eYFP

    Plasmid

    #117383

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV1

    Insert

    EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCE38

    Plasmid

    #174432

    Purpose

    C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCE41

    Plasmid

    #174433

    Purpose

    C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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