We narrowed to 5,822 results for: pCas

1. pAAV-Ef1a-DIO_EGFP-GFE3

   Plasmid

   #79871

   Purpose

   For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-GFE3
   

   Use

   AAV, Cre/Lox, and Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-TSF-TEVcs-ULK1

   Plasmid

   #171416

   Purpose

   For the purifcation of human ULK1 complex

   Depositor

   James Hurley

   Article

   Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

   Insert

   ULK1 (ULK1 Synthetic)
   

   Expression

   Mammalian

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP791_PB_dCas9-SSSavi_BirA

   Plasmid

   #211786

   Purpose

   PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   dCas9, Spy, Snoop, aGCN4, AviTags
   

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   dCas9 D10A and H840A

   Promoter

   pCAGG abd pEF1a

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-PITCh-H2BC11

   Plasmid

   #207755

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. dCas9-10xSunTag(22aa)

   Plasmid

   #215745

   Purpose

   Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   dCas9-10xSunTag
   

   Expression

   Mammalian

   Promoter

   chicken beta-actin promoter

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUDP004

   Plasmid

   #101165

   Purpose

   E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTrichoGate-1

   Plasmid

   #160701

   Purpose

   derivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sites

   Depositor

   Artemio Mendoza

   Article

   Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

   Insert

   lacZ
   

   Use

   Synthetic Biology

   Mutation

   LacZ bordered by BsaI restrction sites

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

   Plasmid

   #232183

   Purpose

   Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6, hUbC

   Available Since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-Amp_CRISPIE donor B1

   Plasmid

   #172842

   Purpose

   CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mEGFP
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHL-FcHis

    Plasmid

    #99846

    Purpose

    mammalian expression with secretive signal and FC tag

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Tags

    Fc_His

    Expression

    Mammalian

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. TC1332

    Plasmid

    #164874

    Purpose

    CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    hAPOBEC3A fused to PspCas13b (APOBEC3A )
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human apobec Y132D

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXYB089

    Plasmid

    #239628

    Purpose

    Plant RNA Vision constructs with no gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01
    stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXYB082

    Plasmid

    #239578

    Purpose

    Plant RNA Vision constructs with 41 bp gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA01
    gRNA02-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXYB083

    Plasmid

    #239579

    Purpose

    Plant RNA Vision constructs with 82 bp gRNAs together with RFP input

    Depositor

    Xiaohan Yang

    Article

    Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

    Inserts

    sfGFPn_Y66-P1_loop_01-stem01-gRNA03
    gRNA04-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
    RFP
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-Aka-P2A-GFP

    Plasmid

    #120867

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    Akaluc
    

    Expression

    Mammalian

    Mutation

    insert Akaluc and a P2A site

    Promoter

    CAG Promoter

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TDP43 NOTAG1

    Plasmid

    #28206

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Full length

    Available Since

    July 1, 2011

    Availability

    Academic Institutions and Nonprofits only

17. CAG-SaCas9-WPRE

    Plasmid

    #89996

    Purpose

    Expresses high levels of WT SaCas9 for genome editing

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SaCas9 (NEWENTRY Staphylococcus aureus)
    

    Tags

    3xHA, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQAT27

    Plasmid

    #223399

    Purpose

    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQAT13

    Plasmid

    #223385

    Purpose

    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    AtUBQ10-hA3A-Y130F-SpCas9-D10A-UGI-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pH-mono-MAD7

    Plasmid

    #170134

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    maize Ubiquitin-1, switchgrass Ubiquitin-1

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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