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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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human PNP non-tagged

Plasmid

#64199

Purpose

Expression of native human PNP (non-tagged)

Depositor

Vern Schramm

Publication

Murkin et al Biochemistry. 2007 May 1;46(17):5038-49.

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Tags

none

Expression

Bacterial

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA3_ASCL1

Plasmid

#64131

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL3 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-CAMKK1

Plasmid

#23824

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMKK1 (CAMKK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-ITGB1BP3

Plasmid

#23393

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ITGB1BP3 (NMRK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-RIOK2

Plasmid

#23535

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIOK2 (RIOK2 Human)

Use

Gateway Cloning

Mutation

C430T, T447A,T453A,1MM

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-PLXNA4B

Plasmid

#23608

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PLXNA4B (PLXNA4 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CAMK1D

Plasmid

#23791

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMK1D (CAMK1D Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Chicken ArcLight A173

Plasmid

#53615

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Publication

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Chicken ArcLight-A173

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRCA422 - pHR-UCOE-SFFV-VPH-dcas9-p2a-mCherry

Plasmid

#238165

Purpose

Express VPH-dCas9-P2A-mCherry in mammalian cells. Lentiviral

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9-P2A-mCherry

Use

CRISPR and Lentiviral

Tags

mCherry

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGS_101

Plasmid

#160541

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Publication

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pHis-hPim1

Plasmid

#100722

Purpose

Expression of human Pim1 kinase domain in E.coli

Depositor

Byung Il Lee

Publication

Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

Insert

Pim1 (PIM1 Human)

Tags

His

Expression

Bacterial

Mutation

Residues 29-313 of isoform 2

Promoter

T7 promotor

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHygromycin (GB0211)

Plasmid

#68204

Purpose

Provides the CDS of the Hygromycin resistance gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hygromycin resistance gene coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-UHMK1

Plasmid

#23433

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

UHMK1 (UHMK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SH3BP5

Plasmid

#23579

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SH3BP5 (SH3BP5 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDONR223-STK32C

Plasmid

#23720

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK32C (STK32C Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTMN140 - UCOE-SFFV-VPH-XTEN-dCas9-IRES2-BFP-T2A-Blast

Plasmid

#238166

Purpose

Express VPH-dCas9 and BFP-T2A-BlasticidinR in mammalian cells. Lentiviral

Depositor

Thomas Norman

Publication

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

VPH-dCas9

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNG-IRES-mScarlet-dMCP

Plasmid

#231089

Purpose

Bicistronic construct encoding mNG in combination with mScarlet-dMCP via an EMCV-IRES sequence.

Depositor

John Ngo

Publication

Kuffner et al Nat Methods. 2025 Sep 22. doi: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mNG-IRES-mScarlet-dMCP

Expression

Mammalian

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPRi

Plasmid

#199808

Purpose

vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

Depositor

Steve Salipante

Publication

Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

dCas9
sgRNA with GFP cassette

Use

CRISPR

Promoter

P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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tetO-GATA6

Plasmid

#170688

Purpose

doxycycline-inducible overexpression of GATA6

Depositor

Paul Burridge

Publication

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

GATA6 (GATA6 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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