We narrowed to 165,343 results for: addgene

1. LentiCRISPRv2-KLF7-1

   Plasmid

   #169797

   Purpose

   Expresses Cas9 and guide RNA for the site neighbouring KLF7 gene.

   Depositor

   Natsuko Chiba

   Article

   Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

   Insert

   guide RNA for KLF7 neighbouring region
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-puro LIP

   Plasmid

   #15713

   Depositor

   Joan Massague

   Article

   Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

   Insert

   LIP (CEBPB Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pAB16 (pmyo-3::QuasAr)

   Plasmid

   #130272

   Purpose

   Expresses the voltage sensor QuasAr in BWMs of C. elegans.

   Depositor

   Alexander Gottschalk

   Article

   Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

   Insert

   QuasAr
   

   Expression

   Worm

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-hSyn-Gβγ-BERKY1

   Plasmid

   #158429

   Purpose

   BRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSyn

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-YFP-GRK3ct
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. AAV-CAG-flex-lck-smV5

   Plasmid

   #196423

   Purpose

   Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Insert

   Lck-smV5
   

   Use

   AAV and Cre/Lox

   Tags

   Lck

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMLV-RT for Split-PE (pEH45)

   Plasmid

   #190105

   Purpose

   Expresses MMLV-RT (full-length, from PE2) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   MMLV-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   RT pentamutant from PE2 (D200N, T306K, W313F, T33…

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

   Plasmid

   #158987

   Purpose

   Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-VP160
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   pMecp2

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Marathon-RT tetramutant for Split-PE (pEH101)

   Plasmid

   #190109

   Purpose

   Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, D74R, N116K, N197R

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-HA-tdMCP-tdTomato

   Plasmid

   #183938

   Purpose

   tandem MS2 binding protein labeled with tdTomato, containing a NLS and a HA-tag

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   tdMCP-tdTomato (cp Synthetic)
   

   Tags

   NLS-HA

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV (TATA box removed)

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. FEN1 C10.3 gRNA

    Plasmid

    #90689

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FEN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FEN1 (Guide Designation C10.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCNL-N1_vinculin

    Plasmid

    #65691

    Purpose

    Expresses Vinculin-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    vinculin (Vcl Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pAGK112

    Plasmid

    #228127

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    

    Expression

    Mammalian

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available Since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SMC6 G4.4 gRNA

    Plasmid

    #90899

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G4.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. SMC6 G3.4 gRNA

    Plasmid

    #90898

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G3.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUW-TetO-mCherry-cNLS

    Plasmid

    #138531

    Purpose

    Lentiviral inducible expression of mCherry with nuclear localization signal

    Depositor

    Ana Teixeira, Christopher Grigsby

    Article

    Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    3x SV40 cNLS

    Expression

    Mammalian

    Promoter

    TRE-CMV

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMX-Flag-WIP

    Plasmid

    #11772

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP (WIPF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. HLCS_pCSdest

    Plasmid

    #53803

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    HLCS (HLCS Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pFH6

    Plasmid

    #86555

    Purpose

    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR; Subcloning

    Promoter

    Arabidopsis U6-26 promoter

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pStr-KDEL_APV-SBP-DsRed2-ER-5

    Plasmid

    #170675

    Purpose

    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Inserts

    APV-SBP-DsRed2-ER-5
    streptavidin-KDEL
    

    Expression

    Mammalian

    Promoter

    IRES and T7

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. FEN1 C9.3 gRNA

    Plasmid

    #90688

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FEN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FEN1 (Guide Designation C9.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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