We narrowed to 13,725 results for: cas9

1. pBC2103-reci

   Plasmid

   #117172

   Purpose

   U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   reci_arCas9 without NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-i53

   Plasmid

   #119002

   Purpose

   Mammalian expression of i53 (53BP1) inhibitor and BFP

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   i53
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AByG

   Plasmid

   #111083

   Purpose

   Integrates marker gene onto D. melanogaster Y chromosome

   Depositor

   Omar Akbari

   Article

   Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

   Inserts

   3xP3 promoter
   tdTomato
   Gypsy insulator
   CTCF insulator
   Y homology arm left
   Y homology arm right
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. sg_shuttle_RFP657

   Plasmid

   #134968

   Purpose

   Lentiviral vector for sgRNA expression

   Depositor

   Beat Bornhauser

   Article

   Huang et al Hemasphere. 2020 Sep 30;4(5):e456. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6-sgRNA; EFS-RFP657

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBLO44.1_dProtease

   Plasmid

   #121631

   Purpose

   CMV-intron_Pro_P2A-mTagBFP2_AmpR_ColE1

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   TEV protease
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken beta-actin promoter

   Available since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. vHB178

   Plasmid

   #193457

   Purpose

   3xHA endogenous tagging of pandoravirus/mollivirus genes

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   3xHA
   

   Use

   Pandoravirus/mollivirus expression

   Tags

   Expression

   Mutation

   Promoter

   ----

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAllet

   Plasmid

   #202560

   Purpose

   E. coli cloning vector with large MCS (50+ unique restriction sites)

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRAU171

   Plasmid

   #86835

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA2

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKER-mKOk

   Plasmid

   #85975

   Purpose

   Expresses mKOk from the EF1a promoter in mammalian cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   mKOk
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAT23

    Plasmid

    #204312

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-GFPc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH1v1

    Plasmid

    #60244

    Purpose

    gRNA expression by the H1 promoter

    Depositor

    Donald Zack

    Article

    Ranganathan et al Nat Commun. 2014 Aug 8;5:4516. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1 promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB8379

    Plasmid

    #126913

    Purpose

    Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and XdCrtYB
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMLS260

    Plasmid

    #73685

    Purpose

    Punc-17::2xNLS-FLP-D5 Expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    Punc-17::2xNLS-FLP-D5::let-858 3'-UTR
    

    Use

    Flp/frt

    Tags

    Expression

    Worm

    Mutation

    G5D

    Promoter

    Punc-17

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMZ283

    Plasmid

    #52224

    Purpose

    Expression of sgRNA precursor with CspCI placeholder for target cloning (see comments).

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRA03

    Plasmid

    #103141

    Purpose

    Triple gRNA expression separated with 28nt Csy4 motif

    Depositor

    Jens Nielsen

    Article

    Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

    Insert

    gRNAs
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB60

    Plasmid

    #47941

    Purpose

    Can be used to generate synthetic guide in vitro from T7 promotor

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    synthetic guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKER-mRuby2

    Plasmid

    #85977

    Purpose

    Expresses mRuby2 from the EF1a promoter in mammalian cells

    Depositor

    Michele Calos

    Article

    Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB8742

    Plasmid

    #126908

    Purpose

    Template for PCR amplification of pGPD1-pFBA1 double promoter biobrick for Yarrowia lipolytica

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    pGPD1 and pFBA1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. AP575-1

    Plasmid

    #71277

    Purpose

    Expresses 3Xflag::tagRFP::myc in bacteria

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

    Insert

    tagRFP
    

    Use

    Tags

    3xFlag and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB8380

    Plasmid

    #126914

    Purpose

    Plasmid containing integration cassette at XII-5 integration site in Saccharomyces cerevisiae for overexpression of XdCrtE

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtE
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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