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Showing: 4841 - 4860 of 66047 results

  1. pGEX-4T-3-GST-Tev-Af1521(K35E/Y145R)-myc

    Plasmid
    #196241
    Purpose
    N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminus
    Depositor
    Robert Sobol
    Publication
    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.
    Insert
    N-terminal GST fusion of Af1521(K35E/Y145R) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
    Tags
    GST tag and myc tag
    Expression
    Bacterial
    Mutation
    amino acid 35 lysine is replaced with glutamic ac…
    Available since
    Aug. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    196241-plasmid-map-sequence-id-398155

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  3. pTM-RBDv2

    Plasmid
    #162785
    Purpose
    SARS-CoV-2 S protein receptor binding domain (RBD) expression in mammalian cells
    Depositor
    Ivan Vorobiev
    Publication
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    SARS-CoV-2 S protein receptor binding domain (RBD) (S Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Tags
    10xHis, c-myc, and hTPA leader
    Expression
    Mammalian
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162785-plasmid-map-sequence-id-333799

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  4. mWasabi-ER-3

    Plasmid
    #56501
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 493, Emission: 509
    Depositor
    Michael Davidson
    Insert
    ER (CALR Human)
    Tags
    mWasabi
    Expression
    Mammalian
    Mutation
    ER Signal Peptide
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  8. EFS-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222497
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222497-plasmid-map-sequence-id-441134

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  11. AID-C12*-dCas9-BE4max

    Plasmid
    #216731
    Purpose
    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.
    Depositor
    Rahul Kohli
    Publication
    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-C12*-dCas9-BE4max (AICDA Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    216731-plasmid-map-sequence-id-429722

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  12. pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196080
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Publication
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Blasticidin S deaminase
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196080-plasmid-map-sequence-id-388988

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  14. pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI

    Plasmid
    #192112
    Purpose
    Expresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Insert
    SARS-CoV-2-S2P-BA.4/5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192112-plasmid-map-sequence-id-374988

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  17. APCS-bio-His

    Plasmid
    #53424
    Purpose
    Expresses full-length Serum amyloid P-component precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    APCS (APCS Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53424-plasmid-map-sequence-id-83212

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Showing: 4841 - 4860 of 66047 results