Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 64,328 results for: %s

Showing: 4841 - 4860 of 64328 results

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pTwist-SARS-CoV-2 Spike-deltaC18 N501Y (TAT)

Plasmid

#212338

Purpose

Encodes SARS-CoV-2 spike N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike N501Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

N501Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 A222V (GTG)

Plasmid

#212329

Purpose

Encodes SARS-CoV-2 spike A222V for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike A222V (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

A222V

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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SL3-MS2-gRNA GG backbone (MOBE3-4)

Plasmid

#219949

Purpose

Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

gRNA-MS2-SL3 (S. pyogenes Cas9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc P

Plasmid

#221855

Purpose

Express human Calreticulin truncated P domain with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Expresses P domain

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc N

Plasmid

#221854

Purpose

Express human Calreticulin truncated N domain with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Expresses N domain

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-CRT-Fc C

Plasmid

#221856

Purpose

Express human Calreticulin truncated C domain with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

Calreticulin (CALR Human)

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Expresses C domain

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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NPS-DuET

Plasmid

#213353

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

NPS (NPS Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgG1

Plasmid

#187010

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L452R-AVI

Plasmid

#176442

Purpose

Expresses SARS-CoV-2 RBD domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-L452R (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452R

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-S477N-AVI

Plasmid

#176443

Purpose

Expresses SARS-CoV-2 RBD domain containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-S477N (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

S477N

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-N501Y-AVI

Plasmid

#176439

Purpose

Expresses SARS-CoV-2 RBD domain containing N501Y mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-N501Y (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

N501Y

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-W152C-AVI

Plasmid

#176431

Purpose

Expresses SARS-CoV-2 NTD domain containing W152C mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-W152C (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

W152C

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-E154K-AVI

Plasmid

#176433

Purpose

Expresses SARS-CoV-2 NTD domain containing E154K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-E154K (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

E154K

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-D614G-AVI

Plasmid

#176324

Purpose

Expresses SARS-CoV-2 S2P protein containing D614G mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-D614G (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

D614G

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDON223 Spike D138Y

Plasmid

#180056

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike D138Y (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike D138Y

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDON223 Spike A570D

Plasmid

#180064

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike A570D (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike A570D

Available Since

Jan. 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike H655Y

Plasmid

#180059

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike H655Y (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike H655Y

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike S982A

Plasmid

#180067

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike S982A (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike S982A

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDON223 Spike T1027I

Plasmid

#180058

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike T1027I (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike T1027I

Available Since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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