We narrowed to 13,035 results for: Mpl

1. pIC306

   Plasmid

   #52257

   Purpose

   Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Article

   Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

   Insert

   Ska3 (SKA3 Human)
   

   Use

   Retroviral

   Tags

   EGFP, S tag, and TEV protease site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS107 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-GFP

   Plasmid

   #199360

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOEu-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-Skp2

   Plasmid

   #19946

   Depositor

   Yue Xiong

   Article

   Michel et al Cell Growth Differ. 1998 Jun . 9(6):435-

   Insert

   S-phase kinase-associated protein 2 (p45) (SKP2 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas9-PCV

   Plasmid

   #123644

   Purpose

   Expresses Cas9 with a C-terminal HUH-tag called PCV2 in E. coli

   Depositor

   Wendy Gordon

   Article

   Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

   Inserts

   PCV2 (ORF1 )
   Cas9
   

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   2-116

   Promoter

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. psiCHECK2-miR-34 WT

   Plasmid

   #78258

   Purpose

   psiCHECK2 dual luciferase reporter harboring a fully complementary miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   fully complimentary miR-34 target site
   

   Use

   Luciferase; Microrna activity

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-FH-eGFP

   Plasmid

   #157734

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged eGFP under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-eGFP
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMALcPP-MS2cp

   Plasmid

   #203351

   Purpose

   Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

   Depositor

   Thomas Cech

   Article

   Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

   Insert

   MS2 coat protein
   

   Use

   Tags

   Hx6-MBP

   Expression

   Bacterial

   Mutation

   V75E and A81G, to prevent capsid assembly

   Promoter

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAPM-D4 miR30-MORC2 ts1

   Plasmid

   #115886

   Purpose

   MORC2 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   MORC2 shRNA (MORC2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTol2 hsp70l loxP-Non-FP-loxP-nCas9n-GFP

   Plasmid

   #158963

   Purpose

   Cre-dependet Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Kesavan et al Development. 2020 Jun 8;147(11). pii: de

   Inserts

   hsp70l promoter
   floxed Non-FP cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid

    #194469

    Purpose

    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

    Depositor

    Mathieu Boulard

    Article

    Formichetti et al Dev Cell 10 Jan 2024

    Insert

    BtGH84
    

    Use

    For in vitro transcription from t3 promoter after…

    Tags

    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-neo-HA-hAtg14

    Plasmid

    #24294

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. RC(WW)-LgBit

    Plasmid

    #208931

    Purpose

    Recognition domain for in vitro MAPK-activity sensor

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    WW domain of PIN1 (PIN1 Human)
    

    Use

    Tags

    HISx6, LgBit, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXWJ109As(RS-te-AAV)

    Plasmid

    #123156

    Purpose

    For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR, PhrpLE

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcGHLT-B-DDB1

    Plasmid

    #48638

    Purpose

    Expresses Full Lenght DDB1 in Insect Cells (ampR)

    Depositor

    Ning Zheng

    Article

    Li et al Cell. 2006 Jan 13;124(1):105-17.

    Insert

    DDB1 (DDB1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

15. EPB104

    Plasmid

    #68369

    Purpose

    contains human U6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    U6 promoter (RNU6-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    U6

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. STAgR_tdTomato

    Plasmid

    #102993

    Purpose

    Can be used as backbone for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Type

    Empty backbone

    Use

    CRISPR; Pcr template for stagr vectors

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRH056

    Plasmid

    #171637

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-tdTomato
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPL5618_pUG_MtxR

    Plasmid

    #60929

    Purpose

    PCR template vector for generating methotrexate resistance cassette.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Dihydrofolate reductase (DHFR Human)
    

    Use

    Loxp flanked deletion cassette template

    Tags

    Expression

    Mutation

    L22F F31S

    Promoter

    TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PROM_MpUBE2

    Plasmid

    #136109

    Purpose

    PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_MpUBE2 Mapoly0106s0019
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. 5UTR_MpUBE2

    Plasmid

    #136110

    Purpose

    5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    5UTR_MpUBE2 Mapoly0106s0019
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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