We narrowed to 10,651 results for: kars;

1. pBK- Ma PylRS WT

   Plasmid

   #197573

   Purpose

   Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Insert

   M. alvus PylRS WT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   GlnS (constitutive)

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFOS WT-GL3

   Plasmid

   #11983

   Purpose

   Reporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.

   Depositor

   Ron Prywes

   Article

   Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

   Insert

   c-fos promoter (Fos Mouse)
   

   Use

   Tags

   Luc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 TAK1/F

   Plasmid

   #44161

   Depositor

   Xin Lin

   Article

   Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

   Insert

   Tak1 (Map3k7 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast POLA1

   Plasmid

   #160807

   Purpose

   Express POLA1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLA1 (POLA1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Codon Optimized

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HAP40 1-371

   Plasmid

   #124060

   Purpose

   Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HAP40 (F8A1 Human)
   

   Use

   Baculovirus expression

   Tags

   6x His and TEV cleavage site

   Expression

   Mutation

   Promoter

   polyhedrin

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. TetO-FUW-MEF2CA

   Plasmid

   #61538

   Purpose

   Expresses MEF2CA in mammalian cells

   Depositor

   Rudolf Jaenisch

   Article

   Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

   Insert

   MEF2CA (MEF2C Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. EF1a_ESRRG_P2A_Hygro_Barcode

   Plasmid

   #120435

   Purpose

   Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   ESRRG (ESRRG Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEGFP-Y705F-STAT3

   Plasmid

   #71445

   Purpose

   Retroviral expression of Y705F-STAT3. Please note that this plasmid contains Y705F-STAT3 tagged with FLAG, not GFP.

   Depositor

   George Stark

   Article

   Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

   Insert

   STAT3 (STAT3 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mutation

   Y705F

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pHAGE-ALDH5A1__MAP3K5

   Plasmid

   #116807

   Purpose

   Lentiviral expression of ALDH5A1__MAP3K5 fusion

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ALDH5A1__MAP3K5
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas-Cas12m

    Plasmid

    #192274

    Purpose

    Encodes MmCas12m under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23108

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6-sgCD71-2

    Plasmid

    #46918

    Purpose

    Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    sgCD71 -2 (TFRC Human)
    Puromycin resistance and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-v2 RsTAL At4CL

    Plasmid

    #126524

    Purpose

    expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

    Depositor

    Andrew Woolley

    Article

    Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

    Inserts

    tyrosine ammonia lyase
    p-coumaroyl-CoA ligase (4CL1 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-hMLH1dn (og codon)

    Plasmid

    #174823

    Purpose

    Mammalian expression of human MLH1dn (original codon usage)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1dn (original codon usage) (MLH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 754-756

    Promoter

    EF1a

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCI-MS2V5-eRF3a F76a

    Plasmid

    #65811

    Purpose

    expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    eRF3a (GSPT1 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    F76a

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. EF1a_SOX2_P2A_Hygro_Barcode

    Plasmid

    #120480

    Purpose

    Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SOX2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBZCas13b

    Plasmid

    #89898

    Purpose

    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. BTG1

    Plasmid

    #155425

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    BTG1 (BTG1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLX302_HOXB13

    Plasmid

    #70089

    Purpose

    Expression of human HOXB13 from CMV promoter, puromycin selection marker

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-EF1A-LivePAR(Y107A)-Hygro

    Plasmid

    #176073

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR (Y107A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation to convert Try107 to Ala

    Promoter

    EF1A

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-SaPE2

    Plasmid

    #174817

    Purpose

    Mammalian expression of SaCas9 prime editor 2

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    SaPE2
    

    Use

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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