We narrowed to 5,570 results for: SPI

1. Cherry-HA-KCC2

   Plasmid

   #104077

   Purpose

   To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

   Depositor

   Igor Medyna

   Article

   Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

   Insert

   KCC2 (b isoform) (Slc12a5 Rat)
   

   Use

   Tags

   mCherry-HA tag

   Expression

   Mammalian

   Mutation

   EcoR1 site in rat KCC2 was removed by silent muta…

   Promoter

   UbC

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

   Plasmid

   #140198

   Purpose

   Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Insert

   dLbCas12a(D156R)-VPR
   

   Use

   CRISPR and Synthetic Biology; Expression in asper…

   Tags

   VPR (VP64-p65-Rta), NLS

   Expression

   Mutation

   D832A DNase deactivated, D156R for improved activ…

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-E55A-ATPIF1

   Plasmid

   #85404

   Purpose

   Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase

   Depositor

   David Sabatini

   Article

   Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

   Insert

   ATPIF1 (ATP5IF1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   E55A point mutation abrogating the ability of ATP…

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pscALPSpuro-RnACE2 (Rat)

   Plasmid

   #158086

   Purpose

   Expresses rat ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Rat ACE2 (Ace2 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FUW-Flag-Mbd3

   Plasmid

   #52371

   Purpose

   Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Ub

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV-AR-C563A-C596A

   Plasmid

   #171220

   Purpose

   Lenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.

   Depositor

   Steven Balk

   Article

   Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

   Insert

   AR-C563A-C596A (AR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-C563A-C596A

   Promoter

   EF-1a

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-sACE2v2.4-8his

   Plasmid

   #149664

   Purpose

   Mammalian expression plasmid for his-tagged sACE2 high affinity variant 2.4

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Short GSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pscALPSpuro-FcACE2 (Cat)

   Plasmid

   #158082

   Purpose

   Expresses cat ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Cat ACE2 (ACE2 )
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. retro-gfp-puro vector

   Plasmid

   #58249

   Purpose

   Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 2XSmBiT-SARS-CoV-2 S1

    Plasmid

    #173430

    Purpose

    protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc

    Depositor

    David Nemazee

    Article

    Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

    Insert

    2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
    

    Use

    Luciferase

    Tags

    human IgG1 Fc

    Expression

    Mammalian

    Mutation

    furin cleavage site RRAR to GSAS

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LV-AR-R599A-N600A

    Plasmid

    #171221

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-R599A-N600A (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-R599A-N600A

    Promoter

    EF-1a

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-sACE2v2.4-sfGFP

    Plasmid

    #149666

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. REV human OCT4 TALEN

    Plasmid

    #52413

    Purpose

    TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Insert

    HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAGGS

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

14. LV-AR-AAFF

    Plasmid

    #171218

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-AAFF (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-K580A-V581A

    Promoter

    EF-1a

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

    Plasmid

    #110300

    Purpose

    Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

    Depositor

    Chris Kok-Lung Chan

    Article

    Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

    Insert

    p53-binding protein 1 (TP53BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-sACE2v1-8his

    Plasmid

    #149269

    Purpose

    Mammalian expression plasmid for his-tagged sACE2 high affinity variant 1

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-sACE2v2.4-IgG1

    Plasmid

    #149668

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.4

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-sACE2v2-sfGFP

    Plasmid

    #145173

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FUW-Mbd3

    Plasmid

    #52356

    Purpose

    Lentiviral expression vector for constitutive expression of Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-sACE2v6-sfGFP

    Plasmid

    #145177

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 6

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge