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C1QBP-bio-His

Plasmid

#53330

Purpose

Expresses full-length Complement component 1 Q subcomponent-binding protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

C1QBP (C1QBP Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-F-Med26-NTD

Plasmid

#49255

Purpose

Mammalian expression of flag-tagged Med26-NTD

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

NTD (aa 1-339)

Available Since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-F-Med26-R61A K62A

Plasmid

#49254

Purpose

Mammalian expression of flag-tagged human Med26 R61A K62A

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

R61A K62A

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pET23a-H6-TEV-cAbl

Plasmid

#214234

Purpose

Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

Depositor

Neel Shah

Article

Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

Insert

ABL1 Kinase domain (ABL1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMURF2_WT

Plasmid

#82259

Purpose

Gateway Donor vector containing SMURF2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMURF2 (SMURF2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pKGmDUET-NgBR/hCIT

Plasmid

#203173

Purpose

Expression of human cis-prenyltransferase complex for purification

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Inserts

NUS1 (NUS1 Human)
DHDDS (DHDDS Human)

Tags

Strep-Tag II-TEV and internally 6HIS tagged

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3 Flrt2-myc

Plasmid

#72192

Purpose

Express full-length Flrt2with a C-terminal myc tag

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt2 (Flrt2 Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

Plasmid

#159575

Purpose

Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

Depositor

Dominic Esposito

Article

Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

Insert

His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)

Tags

His6-tev

Expression

Insect

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK2 ct in pcDNA3.1

Plasmid

#64212

Purpose

mCherry fused between Cry2 and GRK2ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK2 ct (GRK2 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV and cmv

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223_WNT5A_WT

Plasmid

#82240

Purpose

Gateway Donor vector containing WNT5A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WNT5A (WNT5A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHEB_WT

Plasmid

#81257

Purpose

Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHEB (RHEB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 WT

Plasmid

#69819

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_PKIA_WT

Plasmid

#82153

Purpose

Gateway Donor vector containing PKIA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PKIA (PKIA Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBa-KIF5C 559-tdTomato-FKBP

Plasmid

#64211

Purpose

KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Kif5c (Kif5c Rat)

Tags

FKBP and tdTomato

Expression

Mammalian

Mutation

motor domain aa1-559

Promoter

chicken Beta Actin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-WAVE1/SCAR [K91/36R]

Plasmid

#128629

Purpose

Mammalian Expression Plasmid of anti-WAVE1/SCAR (Human). Derived from hybridoma K91/36.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-WAVE1/SCAR (Human) recombinant mouse monoclonal antibody (WASF1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMega-MaFRSA

Plasmid

#200226

Purpose

The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

Depositor

Alanna Schepartz

Article

Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

Inserts

Pyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA

Tags

none

Expression

Bacterial

Mutation

mutated Asparagine 166 to Alanine and Valine 168 …

Promoter

proK-lacO and tacI

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET15-ZmPYK

Plasmid

#220230

Purpose

Inducible expression of Zymomonas mobilis pyruvate kinase

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-TRPS1-P2A-mCherry

Plasmid

#203851

Purpose

Tet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Article

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

TRPS1 (TRPS1 Human)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

Plasmid

#228252

Purpose

pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

Depositor

Benjamin Kleinstiver

Article

Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

Insert

SpCas9 P23H sgRNA, mTagBFP2

Expression

Mammalian

Mutation

n/a

Promoter

CMV and U6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-VGlut2 [N29/29R]

Plasmid

#114501

Purpose

Mammalian Expression Plasmid of anti-VGlut2 (Rat). Derived from hybridoma N29/29.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-VGlut2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a6 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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