Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 146,921 results for: ins;

Showing: 4861 - 4880 of 146921 results

  2. RT-4

    Plasmid
    #220443
    Purpose
    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.
    Depositor
    Marvin Wickens
    Article
    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do
    Insert
    PUF3 (PUF3 Budding Yeast)
    Use
    Tags
    3xHA
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220443-plasmid-map-sequence-id-436941

    Enlarge

  3. Efg1-ΔDBD-A51P/A56P

    Plasmid
    #216400
    Purpose
    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations
    Depositor
    Nicolas Fawzi
    Article
    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0
    Insert
    Efg1-ΔDBD-A51P/A56P
    Use
    Tags
    6xHis-tags
    Expression
    Bacterial
    Mutation
    changed Alanine 51 and 56 to Proline, and deleted…
    Promoter
    Available Since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    216400-plasmid-map-sequence-id-430101

    Enlarge

  4. Efg1-ΔDBD-Q443P

    Plasmid
    #216401
    Purpose
    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation
    Depositor
    Nicolas Fawzi
    Article
    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0
    Insert
    Efg1-ΔDBD-Q443P
    Use
    Tags
    6xHis-tags
    Expression
    Bacterial
    Mutation
    changed Glutamine 443 to Proline, and deleted ami…
    Promoter
    Available Since
    April 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    216401-plasmid-map-sequence-id-430102

    Enlarge

  5. pMAT_41BBICD

    Plasmid
    #197097
    Purpose
    This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for the intracellular domain of 4-1BB.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197097-plasmid-map-sequence-id-386833

    Enlarge

  6. pMAT_CD28ICD

    Plasmid
    #197098
    Purpose
    This plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for the intracellular domain of CD28.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197098-plasmid-map-sequence-id-386835

    Enlarge

  13. pGAL1-VP1 (LEU2)

    Plasmid
    #182480
    Purpose
    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182480-plasmid-map-sequence-id-366129

    Enlarge

  14. mRuby3 only VLP

    Plasmid
    #182429
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-mRuby3
    deltaVP1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182429-plasmid-map-sequence-id-360111

    Enlarge

Showing: 4861 - 4880 of 146921 results