We narrowed to 11,977 results for: pcDNA

1. pcDNA3_HLA-cmyc-GRM3 C127A

   Plasmid

   #140451

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine

   Promoter

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA6 MCV LTco S147A

   Plasmid

   #112192

   Purpose

   Expression of MCPyV LT mutant (Ser 147 to Ala)

   Depositor

   Patrick Moore

   Article

   Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

   Insert

   MCPyV LT codon optimized
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-FRT-TO-FH-Nsp7-Nsp8_GC3opt

   Plasmid

   #157692

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA3-PKAsub-YPet-NES

   Plasmid

   #138203

   Purpose

   Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   PKAsub-YPet-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA pDEST40 Cas9 GW STOP

   Plasmid

   #183196

   Purpose

   Destination vector

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-JNKsubDD-YPet-NES

   Plasmid

   #84633

   Purpose

   Encodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   JNKsubDD-YPet-NES
   

   Use

   Tags

   6xHis, Nuclear export signal (NES), T7 tag (gene …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-hygro-eGFP-HEC1-9D

   Plasmid

   #114029

   Purpose

   Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - 9D (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Hec1 - 9D

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-RIPK1 K163R

   Plasmid

   #78844

   Purpose

   To overexpress RIPK1 K163R in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

   Insert

   RIPK1 (RIPK1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K163R

   Promoter

   CMV

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-TTF-1-AD1-deletion

   Plasmid

   #75265

   Purpose

   Expresses a deletion mutant of human TTF-1 lacking the activation domain 1

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   TTF-1 (NKX2-1 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   AD1 (activation domain 1) deleted

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-TTF-1-AD2-deletion

    Plasmid

    #75266

    Purpose

    Expresses a deletion mutant of human TTF-1 lacking the activation domain 2

    Depositor

    David Mu

    Article

    Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

    Insert

    TTF-1 (NKX2-1 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    AD2 (activation domain 2) deleted

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-FLAG-Lin28B-mCCHC

    Plasmid

    #51375

    Depositor

    Narry Kim

    Article

    Heo et al Cell. 2009 Aug 21;138(4):696-708. doi: 1

    Insert

    Lin28B (LIN28B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    CCHC domain mutant (H137A, H159A)

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA5/FRT/TO GFP DNAJB7

    Plasmid

    #19503

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB7 (DNAJB7 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-5'UTR-MAVS(KaganD88)

    Plasmid

    #52005

    Purpose

    Expresses the human MAVS CDS and contains the 5'UTR sequense

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    5utr-MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5-FRT-TetO-fSNAPc

    Plasmid

    #106986

    Purpose

    open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    fSNAP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 3xFLAG TPP1 88-249

    Plasmid

    #53549

    Purpose

    mammalian expression of TPP1 OB-fold domain

    Depositor

    Kathleen Collins

    Article

    Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    aa 88-249

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1+_LN-HA-AGO2-5xA

    Plasmid

    #92010

    Purpose

    Expresses Lambda-N-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Tags

    Lambda-N-HA

    Expression

    Mammalian

    Mutation

    S824A, S828A, T830A, S831A, S834A (5xA)

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3440 pcDNA3 Bid p22

    Plasmid

    #8774

    Depositor

    Stanley Korsmeyer

    Article

    Zha et al Science 2000 Dec 1;290(5497):1761-5.

    Insert

    Bid p22 wt (Bid Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-PARL-D319A-FLAG C-t

    Plasmid

    #191958

    Purpose

    Expression of Presenilin Associated Rhomboid Like (PARL) D319A mutant

    Depositor

    Luca Scorrano

    Insert

    PARL D319A
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D319A

    Promoter

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/HisMaxB-YAP1-S163A

    Plasmid

    #18989

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Yes-kinase associated protein S163A mutant (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Serine 163 changed to Alanine,

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.2/V5 hsa-mir-499

    Plasmid

    #26320

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-499 (MIR499A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only