We narrowed to 11,231 results for: SHA

1. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

   Plasmid

   #227333

   Purpose

   MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. qTAG-C-Solo-miRFP670nano3-H2BC11

   Plasmid

   #227331

   Purpose

   Donor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. qTAG-N-Solo-mStayGold-TUBA1B

   Plasmid

   #227327

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. qTAG-C-Solo-mStayGold-H2BC11

   Plasmid

   #227330

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. qTAG-C-mStayGold-EFS-Puro-PLK4

   Plasmid

   #227311

   Purpose

   Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. qTAG-N-Solo-mStayGold-PCNT

   Plasmid

   #227285

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PCNT Homology Arms flanking a mStayGold Tag (PCNT Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. siRNA-resistant SigmaR1-mNeonGreen a4mut (I178A L182A L186A V190A)

   Plasmid

   #226577

   Purpose

   Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

   Insert

   SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   I178A, L182A, L186A, V190A

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330-TP53

   Plasmid

   #227318

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of TP53 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEMS2386

   Plasmid

   #225639

   Purpose

   AAV plasmid with Ple388 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Fornes et al Nucleic Acids Res. 2023 Jul 5;51(W1):W37

   Insert

   Ple388 (PITX3 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330-PITCh-MAPRE1

    Plasmid

    #207793

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330-PITCh-MAP4

    Plasmid

    #227295

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MAP4 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of MAP4 (MAP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-EZR

    Plasmid

    #227293

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of EZR (EZR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330-PITCh-CEP192

    Plasmid

    #227288

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CEP192 (CEP192 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330-CEP135

    Plasmid

    #227286

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of CEP135 (CEP135 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330-PITCh-ARL13B

    Plasmid

    #227276

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of ARL13B (ARL13B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3-FLAG-Regnase-3-HA_full-length

    Plasmid

    #222656

    Purpose

    Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG392

    Plasmid

    #226749

    Purpose

    CRISPRi guide RNA against ATP13A1

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    ATP13A1 (ATP13A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG369

    Plasmid

    #226748

    Purpose

    CRISPRi guide RNA against EMC2

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    EMC2 (EMC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG517

    Plasmid

    #226735

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    BCL2L1 (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAG43

    Plasmid

    #226736

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MICU1 (targeting sequence) (MICU1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUC57 mini-FLAG-HA-Regnase-3 WT

    Plasmid

    #222651

    Purpose

    For in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term

    Depositor

    Silvia Monticelli

    Article

    Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

    Insert

    Zc3h12c (Zc3h12c Mouse)
    

    Use

    In vitro transcription

    Tags

    FLAG and HA

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV_RTN3E-mScarlet3

    Plasmid

    #216255

    Purpose

    mammalian expression vector for RTN3E-mScarlet3

    Depositor

    Edward Avezov

    Article

    Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

    Insert

    reticulon 3 isoform e (RTN3 Human)
    

    Use

    Tags

    mScarlet3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHMTc-FBF-2 (121-632)

    Plasmid

    #218664

    Purpose

    Expression of MBP:6xHIS:FBF-2(121-632)

    Depositor

    Judith Kimble

    Article

    Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

    Insert

    fbf-2 (fbf-2 Nematode)
    

    Use

    Tags

    MBP-6xHIS

    Expression

    Bacterial

    Mutation

    amino acids 121-632 only

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-Bcl-2-K17R-K22R

    Plasmid

    #220294

    Purpose

    mammalian expression of Bcl-2-K17R-K22R tagged with GFP

    Depositor

    Xinlai Cheng

    Article

    Wang et al Cell Reports Physical Science, Volume 5,

    Insert

    Bcl2 (BCL2 Human)
    

    Use

    Tags

    Emerald GFP

    Expression

    Mammalian

    Mutation

    K17R-K22R

    Promoter

    CMV

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP-Bcl-2-G128L-A131L-T132L

    Plasmid

    #220304

    Purpose

    mammalian expression of Bcl-2-G128L-A131L-T132L tagged with GFP

    Depositor

    Xinlai Cheng

    Article

    Wang et al Cell Reports Physical Science, Volume 5,

    Insert

    Bcl2 (BCL2 Human)
    

    Use

    Tags

    Emerald GFP

    Expression

    Mammalian

    Mutation

    G128L-A131L-T132L

    Promoter

    CMV

    Available since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJK2097

    Plasmid

    #218665

    Purpose

    Expression of MBP:6xHIS:FBF-2(Y479A, 121-632)

    Depositor

    Judith Kimble

    Article

    Carrick et al Dev Cell. 2024 Mar 11;59(5):661-675.e7.

    Insert

    fbf-2 (fbf-2 Nematode)
    

    Use

    Tags

    MBP-6xHIS

    Expression

    Bacterial

    Mutation

    amino acids 121-632 only, with Y479A mutation

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Dyn1 R271E D291R

    Plasmid

    #198342

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E D291R

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Dyn1 R271E

    Plasmid

    #198343

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Dyn1 R271E E720R

    Plasmid

    #198344

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R271E D291R

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Dyn1 K44A

    Plasmid

    #198340

    Purpose

    Mammalian expression plasmid of GFP-chimera proteins.

    Depositor

    Justin Taraska

    Article

    Jimah et al Dev Cell. 2024 Apr 23:S1534-5807(24)0023

    Insert

    Dynamin1 (DNM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge