We narrowed to 4,893 results for: PIR

1. CMV-SNAP-hCB2_IRES_EGFP

   Plasmid

   #223506

   Purpose

   Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

   Depositor

   James Frank

   Article

   Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

   Insert

   SNAP-tag-CNR2 and EGFP (CNR2 Human, Synthetic)
   

   Tags

   SNAP-tag

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-p300 (D1399Y)

   Plasmid

   #191763

   Purpose

   Expression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   FRB-EGFP-p300 core (D1399Y) (EP300 Human)
   

   Tags

   EGFP (N-terminal of p300 core (D1399Y)) and FRB (…

   Expression

   Bacterial and Mammalian

   Mutation

   catalytic D1399Y mutation in p300

   Promoter

   EF1alpha

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. str-KDEL_Stx5LdeltaER-SBP-mCitrine

   Plasmid

   #154846

   Purpose

   Synchronize trafficking of Stx5LdeltaER (M55V) from the ER, for use with FLIM (RUSH system)

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   Stx5 (Long isoform) (STX5 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCitrine

   Expression

   Mammalian

   Mutation

   Changed Methionine 55 to Valine, causes loss of S…

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPBbleo-TGFa-EREG-ScNeo

   Plasmid

   #209915

   Purpose

   To express the chimeric protein of TGFα and EREG, which a recombinant TGFα protein fused extracellularly to the mScarlet and a recombinant Epiregulin protein fused intracellularly to the mNeonGreen

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   TGFa-EREG-ScNeo (EREG, TGFA Human)
   

   Tags

   GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. str-KDEL_Stx5L(M55V)-SBP-mCitrine

   Plasmid

   #209846

   Purpose

   Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   STX5 (STX5 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCitrine

   Expression

   Mammalian

   Mutation

   Changed Methionine 55 to Valine, causes loss of S…

   Promoter

   CMV

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. str-KDEL_Stx5S-SBP-mCitrine

   Plasmid

   #154845

   Purpose

   Synchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   Stx5 (Short isoform) (STX5 Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and mCitrine

   Expression

   Mammalian

   Mutation

   Lacks amino acids 1-54, enocdes short isoform of …

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGLUE-SHLD2

   Plasmid

   #114118

   Purpose

   Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (SHLD2 Human)
   

   Tags

   HA tag, Streptavidin-binding tag (Streptag), and …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCF806-shABC1

   Plasmid

   #186713

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCF806-shABC2

   Plasmid

   #186714

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid

    #177334

    Purpose

    bacterial expression of active SARS-CoV-2 3C-like proteinase

    Depositor

    Christopher Overall

    Article

    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Factor Xa site-3xFlag-Myc-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid

    #224697

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

    Plasmid

    #177335

    Purpose

    bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

    Depositor

    Christopher Overall

    Article

    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Factor Xa site-3xFlag-Myc-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    Nov. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid

    #224698

    Purpose

    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    

    Tags

    Gly-Gly-6xHis

    Expression

    Bacterial

    Mutation

    silent mutation C to T at position 10546 (elimina…

    Promoter

    T7

    Available Since

    July 2, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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