We narrowed to 7,724 results for: Pol
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Plasmid#196889PurposeDre-activated Cre-inactivated expression of lyn-mScarlet reporter.DepositorInsertlyn-mScarlet
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOET5 rota-VLP
Plasmid#218147PurposeForms rotavirus-like particles in insect cells by expression of VP6 (GenBank ID: ACL93331.1).DepositorInsertRotavirus VP6
ExpressionInsectPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393
Plasmid#216382PurposeBaculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11DepositorPublicationInsertTetraspanin-12 (TSPAN12 Human)
UseBaculovirusTags6xHis-3C-splitGFP11ExpressionInsectPromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Fusion of West Nile NS2B-NS3 proteins
Plasmid#204796PurposeBacterial expression of a fusion of West Nile NS2B-NS3 proteinsDepositorInsertWest Nile NS2B-NS3 fusion
ExpressionBacterialAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac(His10-Shp2)
Plasmid#177899PurposeBaculo expression of His10 tag fused to human Shp2DepositorAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_GRWD1:1-446
Plasmid#210876PurposeInsect expression of full-length human GRWD1. N-terminal 6X His tag with thrombin protease cleavage site.DepositorInsertGRWD1:M1-V446 (GRWD1 Human)
TagsMGSSHHHHHHSSGLVPRGSExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-Dlx-SynaptoTAG
Plasmid#210729PurposeAAV vector for mapping synaptic projections. GABAergic neuron-specific Dlx promoter expresses EGFP-Synaptobrevin-2 to label synaptic terminals and Palm-tdTomato to label neuronal soma and axons.DepositorInsertEGFP-Synaptobrevin-2, P2A, Palm-tdTomato (tdTomato with Palmitoylation sequence) (Vamp2 Synthetic, Rat)
UseAAVPromoterDlxAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1_WT RAD23A
Plasmid#201456PurposeExpresses human RAD23A with an N-terminal GST tag in E. coliDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
TagsGSTExpressionBacterialMutationCoding sequence has been optimised for expression…Promotertac promoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]
Plasmid#196883PurposeDual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporterDepositorInsertroxSTOProx-ZsGreen-bGH
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-lox-roxSTOProx-tRFP-pA-lox
Plasmid#196888PurposeDre-activated Cre-inactivated expression of turbo (t)RFP reporter.DepositorInsertturbo (t)RFP
UseCre/Lox; Dre/roxExpressionMammalianPromoterQuimeric CAGAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-mNeonGreen-CI
Plasmid#196850PurposeExpression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmNeonGreen
ExpressionMammalianMutationNone (wt)PromoterChicken bactin (plus Chicken bactin intron)Available sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-μ2-(HA)3
Plasmid#198178PurposeExpression of HA-tagged AP-2 μ2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAP-2 μ2
Tagstriple HA tagExpressionMammalianMutationsilent substitution in codon 80PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiFi iFE
Plasmid#190141PurposeExpresses HiFiCas9-POLB (HiFi iFE)DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHiFi Cas9-POLB
UseCRISPRTags3xFLAGExpressionMammalianMutationSpCas9 R691A mutant, Codon Optimized for mammalia…PromoterCBhAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T-TEV CHMP1B 1-143
Plasmid#108286PurposeExpresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278DepositorAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJ203
Plasmid#162678PurposepET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)DepositorInsertPutative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
TagsHisExpressionBacterialMutationdeltaF2-A75; codon optimized for expression in E.…PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGBW-m4134347
Plasmid#152890PurposeMammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDF11-SFPQ(369-598)
Plasmid#135438PurposeExpresses SFPQ 369-598 with TEV-cleavable hexahistidine tagDepositorInsertsplicing factor proline and glutamine rich (SFPQ Human)
TagsHexahistidineExpressionBacterialPromoterT7Available sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC177
Plasmid#118886Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease and SNAP tag…ExpressionInsectPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A10-17
Plasmid#112826PurposepSMF2 with all S to A mutations in only repeats 10-17.DepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTD26-S>A18-25
Plasmid#112827PurposepSMF2 with all S to A mutations in only repeats 18-25.DepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationpSMF2 with all S to A mutations in only repeats 1…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only