We narrowed to 7,181 results for: RAP

1. YIp128-CUP1-NUbo-GFP-His6

   Plasmid

   #212790

   Purpose

   Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   NUbo-GFP-His6
   

   Use

   Integrative vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CUP1

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF6 VPS51-V5-6His

   Plasmid

   #175502

   Purpose

   V5 tagged VPS51 is expressed in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Perez-Victoria et al Mol Biol Cell. 2010 Oct 1;21(19):3386-95

   Insert

   VPS51 (VPS51 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   A661V- please see depositor comments

   Promoter

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5 HA-GST-Presc-mAkt1 (wt)

   Plasmid

   #48805

   Purpose

   expresses wt Akt1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Tags

   HA-GST-Presc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

4. TNF-SBP-mCherry_puromycin

   Plasmid

   #65285

   Purpose

   TNF reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-P1-LoxP-Neo-N-miRFP670-C

   Plasmid

   #159434

   Purpose

   CRISPR knock-in donor construct with fluorescent reporter and removable selectable marker

   Depositor

   Jianhua Xing

   Article

   Chen et al Biol Methods Protoc. 2020 Mar 20;5(1):bp

   Inserts

   Neomycin
   miRFP670
   

   Use

   CRISPR

   Tags

   3' linker pair 1, 5' linker pair 1, C t…

   Expression

   Mutation

   Promoter

   SV40

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TOPO tTR-KRAB-rtTA3-Luc

   Plasmid

   #68449

   Purpose

   TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   

   Use

   Luciferase; Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. VanGlow-RR

   Plasmid

   #83343

   Purpose

   for Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

   Depositor

   Cheng-Yu Lee

   Article

   Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   mCherry::Renilla(nls)

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. DHFR-GFP-th-GluA1

   Plasmid

   #173008

   Purpose

   For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   DHFR-GFP-th-GluA1
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZIKV_Trigger_27B

   Plasmid

   #75008

   Purpose

   Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

   Depositor

   James Collins, Alexander Green

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_Trigger_27B
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-DIO-mCherry-WPRE

    Plasmid

    #202620

    Purpose

    Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTriEx-mCherry-LINXa3-nano

    Plasmid

    #73616

    Purpose

    Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa3-nano
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA pDEST40 Cas9-HMGB1

    Plasmid

    #183195

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    HMGB1
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLC-EGFP-RIP1

    Plasmid

    #75163

    Purpose

    LentiCRISPR-EGFP with sgRNA targeting human RIPk1

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Insert

    RIPK1 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXPR_003 sgMYC guide 2

    Plasmid

    #193597

    Purpose

    cMYC knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgMYC guide 2 (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AA007

    Plasmid

    #216002

    Purpose

    Fragmid fragment: (Cas protein) canonical Cas9; NGG PAM

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Cas9_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AA032

    Plasmid

    #216012

    Purpose

    Fragmid fragment: (Cas protein) deactivated Cas

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    dCas9_v1.1 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CoV2-N-D3L

    Plasmid

    #177945

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D3L

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPO685

    Plasmid

    #195300

    Purpose

    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade and native array with PaqCI entry site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose inducible arapBAD

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AA427

    Plasmid

    #215961

    Purpose

    Fragmid fragment: (guide cassette) enables iBar UMIs

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA_DiCre_60.F2

    Plasmid

    #60208

    Purpose

    Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
    

    Use

    Tags

    FRAP and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 2021-2112 of FRAP with a T2098L mutat…

    Promoter

    CMV

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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