We narrowed to 59,446 results for: cloning

1. pJKW1658

   Plasmid

   #119179

   Purpose

   E.coli expression plasmid for Pm4CL1 and PmSPS2

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Inserts

   Pm4CL1
   PmSPS2
   

   Expression

   Bacterial

   Promoter

   pGAP

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH32

   Plasmid

   #125809

   Purpose

   Level0 SpCas9-NG, human codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   SpCas9-NG, human codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. GGE014

   Plasmid

   #120765

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Lip2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   T1_Lip2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p667-UBC-NUAK2-V5-miniTurbo_IDG-K

   Plasmid

   #189910

   Purpose

   Gateway destination clone of NUAK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   NUAK2 (NUAK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pECM1

   Plasmid

   #75445

   Purpose

   Plasmid that serves as the PCR template to create the genomic pdt1 insertions contained in the EPD library. Plasmid contains the R6K origin of replication so needs pir gene to replicate

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   pdt1
   

   Expression

   Bacterial

   Promoter

   none

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCZGY2840

   Plasmid

   #87764

   Purpose

   Pmec-4-PH::miniSOG, expression of membrane targeted PH-miniSOG in mechanosensory neurons

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Pmec-4-PH-miniSOG
   

   Tags

   PH

   Expression

   Worm

   Promoter

   Pmec-4

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GGE257

   Plasmid

   #120771

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Xpr2)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   T2_Xpr2
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCZGY2839

   Plasmid

   #87765

   Purpose

   Prgef-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in neurons.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Prgef-1-PH-miniSOG
   

   Tags

   PH

   Expression

   Worm

   Promoter

   Prgef-1

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRegev_ExonSkip_LandingPad

   Plasmid

   #204186

   Purpose

   Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.

   Depositor

   Oded Regev

   Article

   Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

   Insert

   Beta globin splicing minigene
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   Truncated CAG

   Available Since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFOSGE_COF4C08

    Plasmid

    #71059

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    mael (mael Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pFOSGE_COF1B09

    Plasmid

    #70833

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    rhi (rhi Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

12. MXS_PGK

    Plasmid

    #62414

    Purpose

    MXS_chaining vector with PGK

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    PGK Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-L1-SYN-L4

    Plasmid

    #32583

    Depositor

    Matthew Nolan

    Article

    White et al Front Mol Neurosci. 2011;4:8. Epub 2011

    Insert

    Synapsin I promoter
    

    Use

    Gateway cloning vector

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH58

    Plasmid

    #128184

    Purpose

    Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFH24

    Plasmid

    #131763

    Purpose

    Level0 SpCas9, wheat codon optimized (version 2)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    SpCas9, wheat codon optimized, version 2
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-L1-CAMKII(1.3)-L4

    Plasmid

    #32577

    Depositor

    Matthew Nolan

    Article

    White et al Front Mol Neurosci. 2011;4:8. Epub 2011

    Insert

    CAMKIIalpha promoter 1.3kb version
    

    Use

    Gateway cloning vector

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. P0050::rpaA (KmR)

    Plasmid

    #102321

    Purpose

    drives expression of rpaA from an rpaA-independent promoter (kanamycin resistance casette)

    Depositor

    Erin O'Shea

    Article

    Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

    Insert

    RpaA with p0050 promoter (+ kanamycin resistance casette)
    

    Use

    Cloning vector

    Promoter

    -

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFH92

    Plasmid

    #125820

    Purpose

    Level0 nScCas9-ABE7.10, wheat/human codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    nScCas9-ABE7.10, wheat/human codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRATIO2112

    Plasmid

    #141296

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    mScarlet-I and mVenus

    Expression

    Plant

    Promoter

    UBQ10 promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFH23

    Plasmid

    #128180

    Purpose

    Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::SpCas9 (wheat codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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