We narrowed to 30,626 results for: ica

1. piHGpd/p82

   Plasmid

   #1105

   Depositor

   Susan Lindquist

   Article

   Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

   Insert

   HSP82 (HSP82 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wild-type HSP82 regulated by the strong constitut…

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

2. SepOTSθ

   Plasmid

   #68289

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 2xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBS-AMV-δ1L9'S-nAchR

   Plasmid

   #20687

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   nicotinic acetylcholine receptors
   

   Use

   Tags

   AMV and pA 50

   Expression

   Bacterial

   Mutation

   Leucine at 9' position in M2 region changed …

   Promoter

   Available since

   April 3, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pGBW-m4046181

   Plasmid

   #149090

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pLenti-CMV-SMCHD1-Exon37-48-3xFLAG

   Plasmid

   #130688

   Purpose

   Expresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon37-48 (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   1M_T1522delinsM

   Promoter

   CMV

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-CMV-SMCHD1-Exon1-36-3xFLAG

   Plasmid

   #130681

   Purpose

   Expresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-36 (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D1523_V2005delinsX

   Promoter

   CMV

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGBW-m4046258

   Plasmid

   #148973

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;StrepII

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   May 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4046238

   Plasmid

   #145483

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046187

   Plasmid

   #145403

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 leader protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 leader protein (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. MTK4b_003

    Plasmid

    #123842

    Purpose

    Encodes rabbit beta-globin polyadenylation signal as a Type 4b part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    rglb pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MTK5_011

    Plasmid

    #123855

    Purpose

    Encodes ConR8 as a Type 5 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector R8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. piHGpd/G170D

    Plasmid

    #1113

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with glycine 170 changed to aspartic acid; …

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

13. XE86 TM Delta N Beta-catenin GFP

    Plasmid

    #16833

    Depositor

    Randall Moon

    Article

    Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

    Insert

    TM Delta N beta-catenin GFP (ctnnb1.L Frog)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

14. MTK2_014

    Plasmid

    #123706

    Purpose

    Encodes pUAS (miniCMV core) as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pUAS (mini CMV)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCF226

    Plasmid

    #121667

    Purpose

    EFS-Cas9-wt-P2A-Puro

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a core promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTK4_004

    Plasmid

    #123813

    Purpose

    Encodes spacer sequence as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Spacer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MTK2_018

    Plasmid

    #123710

    Purpose

    Encodes truncated pCMV as a Type 2 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    pCMV-C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Degron-KI PIK3CA donor vector

    Plasmid

    #65621

    Purpose

    Donor vector to generate Degron-KI DD-PIK3CA

    Depositor

    Frank Stegmeier

    Article

    Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

    Insert

    PIK3CA Degron-KI left and right HR arms
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pmECFPC1/GgVcl 1-1066 (CFP at N term)

    Plasmid

    #46281

    Depositor

    Susan Craig

    Article

    Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

    Insert

    Vcl 1-1066 (VCL Chicken)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIS1-mutant Fzd3 3'UTR

    Plasmid

    #25023

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    mutant Fzd3 3'UTR
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    miR-31 binding site in 3’ UTR mutagenized from TC…

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

21. p5Ecldn5bE1

    Plasmid

    #90113

    Purpose

    5' Gateway entry clone containing endothelial specific enhancer sequence of cldn5b

    Depositor

    Nathan Lawson

    Article

    Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

    Insert

    cldn5bE1 (cldn5b Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSicoR-PTPRKi2

    Plasmid

    #59611

    Purpose

    Expression of shRNA against human PTPRK

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    PTPRK (PTPRK Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pSicoR-PTPRKi1

    Plasmid

    #59610

    Purpose

    Expression of shRNA against human PTPRK

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    PTPRK (PTPRK Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pPK720

    Plasmid

    #38147

    Depositor

    Patricia Kuwabara

    Article

    Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

    Insert

    dpy-7p::ptc-3::gfp (ptc-3 )
    

    Use

    Tags

    EGFP

    Expression

    Worm

    Mutation

    Promoter

    dpy-7

    Available since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRC02

    Plasmid

    #114681

    Purpose

    sfGFP.Strand10-Haloenzyme

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Insert

    sfGFP.Strand10-Haloenzyme
    

    Use

    Tags

    His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p3994 pGEM HHR23A

    Plasmid

    #10863

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

    Insert

    HHR23A (RAD23A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

27. pBS C-ret

    Plasmid

    #119900

    Purpose

    Mouse Ret In situ hybridization probe

    Depositor

    David Ginty, Wenqin Luo

    Article

    Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

    Insert

    ret (Ret Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4.1/HisMax-Syx1

    Plasmid

    #35708

    Depositor

    Arie Horowitz

    Article

    Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

    Insert

    Syx1 (Plekhg5 Mouse)
    

    Use

    Tags

    6x HIS and Xpress

    Expression

    Mammalian

    Mutation

    R205K

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

29. 2xDHFR-myc

    Plasmid

    #20215

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xDHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

30. pPK348

    Plasmid

    #38145

    Depositor

    Patricia Kuwabara

    Article

    Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

    Insert

    ptc-3::GFP (ptc-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    ptc-3

    Available since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pIS1-miR-126 site

    Plasmid

    #25026

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    synthetic miR-126 site
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pSicoR-TTF1i2

    Plasmid

    #59621

    Purpose

    Expression of shRNA against human TTF1

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    TTF1 (TTF1 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pQCXIP-Laforin Myc R171H

    Plasmid

    #84671

    Purpose

    mammalian expression and/or retrovirus preparation

    Depositor

    Ayano Satoh

    Article

    Toyota et al Sciencematters

    Insert

    Laforin (EPM2A Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

34. pPD_Pinx-1::G-CaMP3

    Plasmid

    #117422

    Purpose

    For Calcium imaging, expression of G-CaMP3 under the expression of a Pinx-1 promoter (AIB interneurons)

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    G-CaMP3
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    inx-1

    Available since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pESC-LEU2-RAD30cmyc

    Plasmid

    #60821

    Purpose

    to overexpress yeast polymerase eta in yeast

    Depositor

    Thomas Kunkel, Youri Pavlov

    Article

    Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

    Insert

    RAD30 (RAD30 Budding Yeast)
    

    Use

    Tags

    c-myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

36. p067 pCS mTRPS1 P4

    Plasmid

    #11039

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

    Insert

    TRPS1 P4 (Trps1 Mouse)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Truncation at aa1121 to mimic frameshift at codon…

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

37. T7-AglC-His6

    Plasmid

    #89726

    Purpose

    Expresses AglC from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    AglC
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. T7-AglK-His

    Plasmid

    #89714

    Purpose

    Expresses AglK from M. voltae with an N-terminal T7 tag and a C-terminal His6 tag. Plasmid has codons optimized for E. coli expression.

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    AglK
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. His8-TEV-PseC

    Plasmid

    #89724

    Purpose

    Expresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage site

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    PseC (pseC Campylobacter jejuni)
    

    Use

    Tags

    His8 and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

40. GST-PglF130

    Plasmid

    #89708

    Purpose

    Expresses truncated PglF from C. jejuni with N-terminal GST tag

    Depositor

    Barbara Imperiali

    Article

    Zamora et al Methods Enzymol. 2017;597:145-186. doi:

    Insert

    pglF
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 1-129 of putative transmembrane domain h…

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge