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MO70-hJunctate - EF-mutant

Plasmid

#79597

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

Insert

junctate (ASPH Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

77DAD79 to 77AAA79

Promoter

CMV

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
PRK5-FLAG-USP13/5-4

Plasmid

#61746

Purpose

express N-terminally FLAG tagged human chimera USP13/5-4

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-4 chimera (USP5, USP13 Human)

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-716aa) fused to USP13 (722-864aa)

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-6

Plasmid

#61748

Purpose

express N-terminally FLAG tagged human chimera USP13/5-6

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-6 chimera (USP5, USP13 Human)

Tags

FLAG

Expression

Mammalian

Mutation

USP13 (1-721aa) fused to USP5 (717-859aa); D23Y i…

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-5

Plasmid

#61747

Purpose

express N-terminally FLAG tagged human chimera USP13/5-5

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-5 chimera (USP5, USP13 Human)

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-781aa) fused to USP13 (786-864aa); G2667A…

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-USP13/5-3

Plasmid

#61745

Purpose

express N-terminally FLAG tagged human chimera USP13/5-3

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP13/5-3 chimera (USP5, USP13 Human)

Tags

FLAG

Expression

Mammalian

Mutation

USP5 (1-628aa) fused to USP13 (653-864aa)

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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MO70-hCRACR2A-isoform-c

Plasmid

#79591

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Nat Cell Biol. 2010 May;12(5):436-46. do

Insert

CRACR2A-isoform-c (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
MO70-N-Flag-hCRACR2A-isoform-a-Q604L

Plasmid

#79595

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Q604L

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
MO70-N-Flag-hCRACR2A-isoform-a-T559N

Plasmid

#79594

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

T559N

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
Human NCKX4-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75212

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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RF-211-UB-Lyn

Plasmid

#79184

Purpose

Expression of the C-terminal fragment of Lyn kinase that is endogenously generated by caspase cleavage in mammalian cells

Depositor

Richard Fahlman

Article

Eldeeb et al Oncotarget. 2014 May 15;5(9):2714-22.

Insert

Lyn (LYN Human)

Tags

3x Flag and ubiquitin

Expression

Mammalian

Mutation

delta 1-18

Promoter

CMV

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 494-1216 (USP)

Plasmid

#78588

Purpose

Expression flag-USP19 494-1216 (USP) in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1216 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1216 residue…

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1290

Plasmid

#78580

Purpose

Expression flag-USP19 494-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1290 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1291 residue…

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RIPK1 K163R

Plasmid

#78844

Purpose

To overexpress RIPK1 K163R in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK1 (RIPK1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K163R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-RIPK3 4KR

Plasmid

#78821

Purpose

To overexpress RIPK3 4KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K55R, K89R, K363R, K501R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIB Flag MFF S155,172A

Plasmid

#74387

Purpose

Retroviral expression vector containing Flag tagged S155,172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Ser 155 Ala, Ser 172 Ala (Numbering based on MFF …

Promoter

CMV

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only
pECE M2-SH3PXD2A 6 serines mutated to alanines

Plasmid

#69815

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A S974A

Plasmid

#69814

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SNX17 S437A

Plasmid

#69812

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIB Flag MFF WT

Plasmid

#74384

Purpose

Retroviral expression vector containing Flag tagged WT MFF isoform 5

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM1

Plasmid

#72208

Purpose

Mammalian expression of aa 1-372 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Δ 373-860

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only