We narrowed to 17,703 results for: puromycin
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Plasmid#185768PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, AIDMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_Z18-PDGFR_EF1a_eGFP
Plasmid#186304PurposePlasmid for inducible mammalian cell expression of the Z18 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction.DepositorInsertsZ18-PDGFR
eGFP
TagsHA, mycExpressionMammalianAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO‐puro‐2A‐mdx4cv‐GFP
Plasmid#187053Purposefluorescent reporter for adenine base editing of mdx4cv point mutationDepositorInsertGFP
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9
Plasmid#186699PurposePlasmid encoding PRDM9dC-Cas9 fusion under CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
UseCRISPRTagsFLAGExpressionMammalianPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11440
Plasmid#183203PurposepHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPREDepositorInserthyperdCas12 base editor
UseCRISPR and LentiviralAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLPC-FLAG.Mm.KAT5
Plasmid#184647PurposeRetroviral expression of mouse Kat5DepositorAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-HypaCas9-mRuby2
Plasmid#183872PurposepX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.DepositorTypeEmpty backboneUseCRISPRTagsHypaCas9-P2A-mRuby2-T2A-PuroExpressionMammalianPromoterCbhAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTAGBFP2 NES visual barcode
Plasmid#158667Purposetagging clonal populations with NES mTAGBFP2 fluorescent protein. Adding NES to mTAGBFP2 WCDepositorInsertmTAGBFP2
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_SPTLC1_C133W
Plasmid#181921Purpose3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133WDepositorInsertHuman serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
UseLentiviral; Mammalian expression, , doxycycline i…ExpressionMammalianMutationcysteine at position 133 of the coding region of …Promotertight TRE promoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro PRMT5-CRISPRi
Plasmid#164637PurposelentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2DepositorInserttarget guide sequence PRMT5 inhibition (PRMT5 Human)
UseCRISPR and Lentiviral; InterferenceExpressionMammalianPromoterU6 and EF1AAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiCrisprV2 sgRNA hCRY1 c-term
Plasmid#179453PurposeLentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-inDepositorInsertsgRNA targeting human CRY1
UseCRISPR and LentiviralPromoterhU6Available sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZR064_CROPseq-sgCD4-CRISPRi
Plasmid#180270PurposeLentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRiDepositorInsertCD4 sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE SpRY
Plasmid#179082PurposeNOTE: An improved version of this plasmid is now available. See Addgene plasmid #235998. All-in-one prime editor piggyBac transposon, SpRY variantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationA61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…PromoterCAGAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFS-ccdB-12X MS2_mPGK-Puro
Plasmid#177809PurposeAssembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'DepositorTypeEmpty backboneTags12X MS2 stem loopsExpressionMammalianPromoterEFSAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_sgCtrl#2
Plasmid#174149PurposeLentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPB-TRE-ECC-Biosensor
Plasmid#140833PurposeEncoding the TRE promoter based eccDNA biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTRE-ECC Biosensor
UsePiggybac (pb) transposonExpressionMammalianPromoterinverseAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-Pur LaG17-synNotch-TetRVP64
Plasmid#158133PurposeConstitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backboneDepositorInsertLaG17-synNotch-TetRVP64
UseTransposonExpressionMammalianPromoterEF1αAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEX305-N-dTAG-CARD8
Plasmid#169989PurposeMammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)DepositorAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1-S259A
Plasmid#131727PurposeMammalian Expression of RAF1DepositorAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEXPqcxip-hCCDC47-FLAG
Plasmid#159141PurposeHu CCDC47 -flag mammalian expression Gateway vector.DepositorInsertcoiled-coil domain containing 47 (CCDC47 Human)
UseRetroviralTagsFLAGExpressionMammalianPromoterCMV (cytomegalovirus) enhancer and the MSV (mouse…Available sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only