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CBM9-ID-F

Plasmid

#171639

Purpose

Expresses SARS-CoV-2 Spike epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Spike protein (S SARS-CoV-2)

Tags

CBM-9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CBM9-ID-H1

Plasmid

#171709

Purpose

Expresses SARS-CoV-2 Spike epitope

Depositor

Francis Nano

Article

McGuire et al Microb Cell Fact. 2022 Feb 5;21(1):21. d

Insert

SARS-CoV-2 Spike protein (S SARS-CoV-2)

Tags

CBM-9

Expression

Bacterial

Available Since

July 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pHL 1917

Plasmid

#53024

Purpose

AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Inserts

mCherry
fimB (fimB E. coli)

Use

Synthetic Biology

Tags

Asp terminator, RBS(st7), and T1 terminator

Expression

Bacterial

Promoter

pCon and pLlacO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pJOE6089.4

Plasmid

#135081

Purpose

pBR322-based L-rhamnose-inducible vector.

Depositor

Josef Altenbuchner

Article

Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

Insert

P-rhaP-BAD-eGFP-Strep-tag II

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMiNGo11-3

Plasmid

#126058

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

mitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMiNGo11-7

Plasmid

#126057

Purpose

A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

mitochondria Rieske iron sulphur protein (1-100 aa)
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)

Use

Binary

Tags

GFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJ02B2Gm:Rm_AF

Plasmid

#66061

Purpose

MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Rm:Gm_AF

Plasmid

#66062

Purpose

MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pA_higher-CheY-cpAsLOV2_N449S

Plasmid

#253259

Purpose

Encodes the fusion of CheY and cpAsLOV2 N449S driven with the proA promoter and wild type T7 RBS

Depositor

Aravinthan DT Samuel

Article

Vrabioiu et al Proc Natl Acad Sci U S A. 2026 Mar 10;12

Insert

CheY-cpAsLOV2_N449S

Expression

Bacterial

Mutation

wild type T7 RBS

Available Since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pET-28b(+)_N-His_NEDD8

Plasmid

#221866

Purpose

Expresses mature WT human NEDD8 with a HIS tag in E. coli

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

NEDD8 (NEDD8 Human)

Tags

6x His - thrombin cleavage site

Expression

Bacterial

Mutation

Codon optimised for expression in E. coli

Promoter

T7

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_rTetR-VP16 (3'UTR)_RLEloop

Plasmid

#240257

Purpose

Attenuated PROTEUS vector with rtTA-3G transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

rTetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_TetR-VP16 (3'UTR)_RLEloop

Plasmid

#240259

Purpose

Attenuated PROTEUS vector with tTA transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

TetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pBEL3250

Plasmid

#247058

Purpose

Genetic complementation plasmid of LetB(R63K) with LetA

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST

Expression

Bacterial

Mutation

LetA R63K

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pBEL3287

Plasmid

#247059

Purpose

Expression plasmid of of LetA with LetB(W476B)

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST

Expression

Bacterial

Mutation

LetB W476Amber

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pBEL3288

Plasmid

#247060

Purpose

Expression plasmid of of LetA with LetB(Y814B)

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST

Expression

Bacterial

Mutation

LetB Y814Amber

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pBEL2637

Plasmid

#247061

Purpose

Expression plasmid of of LetA with LetB(F833B)

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST

Expression

Bacterial

Mutation

LetB F833Amber

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pBEL2937

Plasmid

#247062

Purpose

Expression plasmid of of LetA with LetB(K488B)

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST

Expression

Bacterial

Mutation

LetB K488Amber

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pSGAb/pEvol-AbTrpRS(H14)

Plasmid

#231133

Purpose

Expresses mutant A. baumannii TrpRS from an inducible lac promoter for proteome-wide incorporation of tryptophan analogues.

Depositor

Abhishek Chatterjee

Article

Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/

Insert

A. baumannii tryptophanyl tRNA synthetase

Use

Acinetobacter baumannii expression

Expression

Bacterial

Mutation

T12A, V151G, V153C

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBN201

Plasmid

#217917

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations

Depositor

James Naismith

Article

Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E mutations

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y715E, Y717E, Y718E

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBN363

Plasmid

#217918

Purpose

express E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutations

Depositor

James Naismith

Article

Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:

Insert

E. coli E69 (O9a:K30) Wzc_K540M with Y715E, Y717E, Y718E and N711Y mutations

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

K540M, Y715E, Y717E, Y718E, N711Y

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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