We narrowed to 7,926 results for: AMPH

1. pCas12b(PI)

   Plasmid

   #186451

   Purpose

   Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12b (PI)
   

   Use

   CRISPR

   Mutation

   R122A, G143P

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnYC-NvM-DmBam_1-140_AG

   Plasmid

   #148832

   Purpose

   Bacterial Expression of DmBam_1-140

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

   Insert

   DmBam_1-140 (bam Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pnYC-NpM-DmNot1_1468-1719_AF

   Plasmid

   #148740

   Purpose

   Bacterial Expression of DmNot1_1468-1719

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

   Insert

   DmNot1_1468-1719 (Not1 Human)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnYC-NpM-DmRoquin_702-819-del790-812-GB1His6_AE

   Plasmid

   #148623

   Purpose

   Bacterial Expression of DmRoquin_702-819-del790-812

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

   Insert

   DmRoquin_702-819-del790-812 (roq Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnYC-NpM-DmRoquin_702-819-del725-755-GB1His6_AE

   Plasmid

   #148622

   Purpose

   Bacterial Expression of DmRoquin_702-819-del725-755

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

   Insert

   DmRoquin_702-819-del725-755 (roq Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pnYC-NpM-HsNot1iso1_1833-2361_U

   Plasmid

   #147728

   Purpose

   Bacterial Expression of HsNot1iso1_1833-2361

   Depositor

   Elisa Izaurralde

   Article

   Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

   Insert

   HsNot1iso1_1833-2361 (CNOT1 Human)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnYC-NvH-HsNot1iso1_1565-2371_U

   Plasmid

   #147730

   Purpose

   Bacterial Expression of HsNot1iso1_1565-2371

   Depositor

   Elisa Izaurralde

   Article

   Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

   Insert

   HsNot1iso1_1565-2371 (CNOT1 Human)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pnYC-NvHN-DmXRN1_1312-1383_N

   Plasmid

   #147051

   Purpose

   Bacterial Expression of DmXRN1_1312-1383

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   DmXRN1_1312-1383 (pcm Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pnYC-NvHN-DmXRN1_1323-1383_N

   Plasmid

   #147053

   Purpose

   Bacterial Expression of DmXRN1_1323-1383

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

   Insert

   DmXRN1_1323-1383 (pcm Fly)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnYC-NpH-CeeIF4E3_1-215opt-V58AI74A_AB

    Plasmid

    #148368

    Purpose

    Bacterial Expression of CeeIF4E3_1-215-V58AI74A

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

    Insert

    CeeIF4E3_1-215-V58AI74A
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnYC-NpM-HsNot1iso1_1833-2361-H1949D_AB

    Plasmid

    #148335

    Purpose

    Bacterial Expression of HsNot1iso1_1833-2361-H1949D

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    HsNot1iso1_1833-2361-H1949D (CNOT1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pnYC-NpM-HsNot1iso1_1833-2361-V1880E_AB

    Plasmid

    #148336

    Purpose

    Bacterial Expression of HsNot1iso1_1833-2361-V1880E

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    HsNot1iso1_1833-2361-V1880E (CNOT1 Human)
    

    Expression

    Bacterial

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRB116.2

    Plasmid

    #181946

    Purpose

    aTc-inducible expression of TorR with C-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    TorR-mNG; mCherry; tetR (AX04_RS23485, torR torR - E. coli, Synthetic)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Promoter

    TorR-mNG-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRB120.2

    Plasmid

    #181947

    Purpose

    aTc-inducible expression of TorR with N-terminal mNeonGreen fusion. Also contains mCherry under TorR-controlled promoter PtorCAD129

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-TorR; mCherry; tetR (torR, AX04_RS23485 torR - E. coli, Synthetic)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Promoter

    mNG-TorR-PLtetO-1; mCherry-PtorCAD129; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRB133.2

    Plasmid

    #181950

    Purpose

    aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 PhoP-Salmonella enterica subsp. enterica serovar Typhimurium, Synthetic)
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Promoter

    PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPbuCas13b-gRNA-1

    Plasmid

    #184567

    Purpose

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBK.136

    Plasmid

    #187220

    Purpose

    Expresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectively

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Insert

    Retron Eco1 ncRNA v35 (long a1/a2); Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
    

    Expression

    Bacterial

    Promoter

    A: pSalTTC; B: pTet*

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

    Plasmid

    #186413

    Purpose

    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ E. coli)
    

    Use

    Gateway destination vector

    Tags

    Nuclear localization signal

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmRE-Tn5-142

    Plasmid

    #118531

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWP001

    Plasmid

    #178596

    Purpose

    pAF256 derived plasmid encoding a xylose inducible CD16/50L CBD-SmBiT and LgBiT

    Depositor

    Robert Fagan

    Article

    Phothichaisri et al Microbiol Spectr. 2022 Apr 4:e0236121. d

    Insert

    CBD-SmBiT/LgBiT
    

    Expression

    Bacterial

    Promoter

    Pxyl

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pnYC-NvHN-DmXRN1_1323-1355_N

    Plasmid

    #147052

    Purpose

    Bacterial Expression of DmXRN1_1323-1355

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

    Insert

    DmXRN1_1323-1355 (pcm Fly)
    

    Expression

    Bacterial

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCASCADE-gltA1-gltA2

    Plasmid

    #71348

    Purpose

    gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Article

    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

    Insert

    gltA1-gltA2 guide array
    

    Expression

    Bacterial

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. CDS12_Cas9-NLS

    Plasmid

    #136122

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_AtcoCas9-NLS(noStop)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pEPQD0CM0065

    Plasmid

    #177019

    Purpose

    Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    1-deoxy-D-xylulose 5-phosphate synthase
    

    Use

    Synthetic Biology

    Mutation

    transit peptide removed, amplified from cDNA, con…

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTF101.1gw3

    Plasmid

    #172181

    Purpose

    A binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

    Depositor

    Kan Wang

    Article

    Paz et al Plant Cell Rep. 2006 Mar;25(3):206-13. d

    Insert

    attR3-CmR-ccdB-attR4
    

    Expression

    Plant

    Promoter

    lacUV5 promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. PdnaK-IR2-IR2 GFP pZa

    Plasmid

    #170083

    Purpose

    GFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosis

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PdnaK-IR2-IR2

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pUPD2 AtU6-26:AsCas12a_DR (GB1442)

    Plasmid

    #160574

    Purpose

    AsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtU6-26:AsCas12a_DR
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUPD2 LbCas12a (GB1439)

    Plasmid

    #160573

    Purpose

    Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    LbCas12a
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUPD2 AsCas12a (GB1438)

    Plasmid

    #160572

    Purpose

    Human codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. IBMc367

    Plasmid

    #161947

    Purpose

    Contains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
    

    Expression

    Bacterial

    Available Since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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