We narrowed to 18,127 results for: Mut

1. pEPkan-S

   Plasmid

   #41017

   Purpose

   PCR template for en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   Kana_I-SceI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-PSmOrange2

   Plasmid

   #34957

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2012 Aug 17.

   Insert

   PSmOrange2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R17H, R36H, F64I, Q194L, A224S, G226A (mutations …

   Promoter

   Available Since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Flag-Hsp40-S171A

   Plasmid

   #84999

   Purpose

   Hsp40/DnaJB1 expression plasmid

   Depositor

   Ugo Moens

   Insert

   Hsp40 (DNAJB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Serine 171 mutated to Alanine

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pEPkan-S2

   Plasmid

   #61601

   Purpose

   PCR template for en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   Kana_I-SceI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. inactive cdk1 FRET sensor (2328)

   Plasmid

   #26065

   Depositor

   Jonathon Pines

   Article

   Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

   Insert

   Cdk1 FRET Sensor- Inactive
   

   Use

   Tags

   CFP variant, FLAG, and YFP variant

   Expression

   Mammalian

   Mutation

   Ser 126 mutated to Ala

   Promoter

   Available Since

   Sept. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pT2/SVNeo

   Plasmid

   #26553

   Depositor

   Perry Hackett

   Article

   Cui et al J Mol Biol. 2002 May 17. 318(5):1221-35.

   Insert

   pT2/SVNeo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   added the Rmut(80, 87, d101)

   Promoter

   Available Since

   March 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Casp2 C303A-Flag

   Plasmid

   #11812

   Depositor

   Guy Salvesen

   Insert

   Caspase-2 catalytic mutant (CASP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C303A

   Promoter

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pAc5.1B-EGFP-DmAtx2_AE

   Plasmid

   #148548

   Purpose

   Insect Expression of DmAtx2

   Depositor

   Elisa Izaurralde

   Insert

   DmAtx2 (Atx2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Three silent mutations and two deletions compared…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-EC12WTC9AAvi

   Plasmid

   #47925

   Purpose

   Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

   Depositor

   Tanja Kortemme

   Article

   Ritterson et al J Am Chem Soc. 2013 Aug 7.

   Insert

   E-Cadherin EC12
   

   Use

   Tags

   6xHis, Avi Tag, and TEV Cleavage

   Expression

   Bacterial

   Mutation

   changed Cysteine 9 to Alanine

   Promoter

   T7

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C054

    Plasmid

    #174342

    Purpose

    Template for making Fluc mRNA reporter with pA124 and mutant mouse Malat1.

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    Firefly luciferase
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCM121

    Plasmid

    #220833

    Purpose

    EryA sensor with increased sensitivity (mphA and mphR (T17R) mutation) and high level resistance (ermC)

    Depositor

    Matthew Bennett

    Article

    Miller et al ACS Synth Biol. 2021 Feb 19;10(2):258-26

    Insert

    mphR
    

    Use

    Tags

    Expression

    Mutation

    T17R

    Promoter

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLNHA-C1-HsNot1iso1-shRNAres_V

    Plasmid

    #147749

    Purpose

    Mammalian Expression of HsNot1iso1-shRNAres

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1iso1-shRNAres (CNOT1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1 silent mutation compared to the sequence of iso…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pREMI-Z

    Plasmid

    #59527

    Purpose

    Zeocin-resistance plasmid for insertional mutagenesis.

    Depositor

    Benjamin Glick

    Article

    Mukaiyama et al Genes Cells. 2002 Jan;7(1):75-90.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-Mcm2-mVen

    Plasmid

    #211398

    Purpose

    mammalian expression of human Mcm2 C-terminally fused to mVenus

    Depositor

    Helmut Pospiech

    Insert

    Mcm2 (MCM2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-EGFP-C1-HseIF4G_O

    Plasmid

    #147091

    Purpose

    Mammalian Expression of HseIF4G

    Depositor

    Elisa Izaurralde

    Insert

    HseIF4G (EIF4G1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V compared to the seu…

    Promoter

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Ro1

    Plasmid

    #24504

    Depositor

    Bruce Conklin

    Insert

    RASSL Ro1 (OPRK1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    mutations in extracellular loop 2, making it a RA…

    Promoter

    Available Since

    June 25, 2010

    Availability

    Academic Institutions and Nonprofits only

17. HiFi iFE

    Plasmid

    #190141

    Purpose

    Expresses HiFiCas9-POLB (HiFi iFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    HiFi Cas9-POLB
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    SpCas9 R691A mutant, Codon Optimized for mammalia…

    Promoter

    CBh

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBlueScript NCX1.1

    Plasmid

    #66799

    Depositor

    Michela Ottolia, Kenneth Philipson

    Article

    Nicoll et al Science. 1990 Oct 26;250(4980):562-5.

    Insert

    NCX1.1
    

    Use

    T3 transcription of gene

    Tags

    Expression

    Mutation

    there are 3 silent mutations within the open read…

    Promoter

    T3

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJK276

    Plasmid

    #73366

    Purpose

    Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)

    Depositor

    T. J. Kappock

    Insert

    plastid omega-3 fatty acid desaturase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Mutation I231F. Also insert is missing residues 1…

    Promoter

    GAL1

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCellFree_KA1_SARS-COV-2_RBD -N501Y

    Plasmid

    #169407

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 mutated RBD domain (N510Y, UK strain) of S protein with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _RBD (N501Y) (S SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    N501Y

    Promoter

    T7- CMV/CAG

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. MLH1

    Plasmid

    #28120

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLH1:JMC01L-F09:C205690 (MLH1 Human)
    

    Use

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-347.

    Promoter

    Available Since

    March 21, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pHBS1051 TDP43 RRM-GFP F-Y

    Plasmid

    #107802

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All phenylalanine to tyrosine in LLPS reporter

    Promoter

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHBS932 TDP43 RRM-GFP Φ-S

    Plasmid

    #107798

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobics to serine

    Promoter

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT7-7 asyn WT1-106 Mxe

    Plasmid

    #105746

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein 1-106 Mxe (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    1-106AA Mxe

    Promoter

    T7

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET11a asyn Q109E (TAC-->TAT)

    Plasmid

    #105735

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q109E (TAC-->TAT) (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q109E

    Promoter

    T7

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-7 asyn K32R/K34R

    Plasmid

    #105741

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein K32R/K34R (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K32R/K34R

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET11a asyn Q109N (TAC-->TAT)

    Plasmid

    #105737

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q109N (TAC-->TAT) (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q109N

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET11a asyn Q99E (TAC-->TAT)

    Plasmid

    #105734

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q99E (TAC-->TAT) (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q99E

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET11a asyn Q79E (TAC-->TAT)

    Plasmid

    #105732

    Purpose

    Bacterial expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Insert

    alpha-synuclein Q79E (TAC-->TAT) (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Q79E

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HA-mRIPK3 T231A/S232A-Flag/cHA-pTRIPZ vector

    Plasmid

    #73707

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    T231A/S232A

    Promoter

    CMV/tet

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

31. HA-mRIPK3 AAAA-Flag/cHA-pTRIPZ vector

    Plasmid

    #73702

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    448-AAAA-451

    Promoter

    CMV/tet

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pJK142

    Plasmid

    #73317

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tag

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Use

    Tags

    His6, Pas2p, and c-myc

    Expression

    Yeast

    Mutation

    synthetic gene. Encodes a glycine-204 to serine m…

    Promoter

    AOX1

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. TRPML1 V432P mScarlet-I

    Plasmid

    #245041

    Purpose

    Expresses TRPML1 mutant with C-terminus mScarlet-I tag in mammalian cells

    Depositor

    Thomas Martin

    Insert

    TRPML1 (MCOLN1 Human)
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    V432P

    Promoter

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Ec FkP-dnaN(3E's)-pRSFDuet

    Plasmid

    #238344

    Purpose

    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli

    Depositor

    Michael O'Donnell

    Insert

    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)
    

    Use

    Tags

    3X FLAG+PKA kinase motif+PreScission Protease cle…

    Expression

    Bacterial

    Mutation

    R96E; R103E; R105E

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Ec FkP-dnaN(3A's)-pRSFDuet

    Plasmid

    #238343

    Purpose

    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli

    Depositor

    Michael O'Donnell

    Insert

    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A) (dnaN E. coli)
    

    Use

    Tags

    3X FLAG+PKA kinase motif+PreScission Protease cle…

    Expression

    Bacterial

    Mutation

    R96A; R103A; R105A

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ec FkP-dnaN(3S's)-pRSFDuet

    Plasmid

    #238345

    Purpose

    over-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coli

    Depositor

    Michael O'Donnell

    Insert

    PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96S; R103S; R105S) (dnaN E. coli)
    

    Use

    Tags

    3X FLAG+PKA kinase motif+PreScission Protease cle…

    Expression

    Bacterial

    Mutation

    R96S; R103S; R105S

    Promoter

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TDP-43 K181E GFP

    Plasmid

    #237549

    Purpose

    Expression of GFP-tag fused to mutant TDP-43 K181E

    Depositor

    Tatyana Shelkovnikova

    Insert

    GFP-tagged TDP-43 K181E (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TDP-43 K181E

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TDP-43 K181A GFP

    Plasmid

    #237550

    Purpose

    Expression of GFP-tag fused to mutant TDP-43 K181A

    Depositor

    Tatyana Shelkovnikova

    Insert

    GFP-tagged TDP-43 K181A (TARDBP Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TDP-43 K181A

    Promoter

    CMV

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pB-T-PAF-Clu CC4

    Plasmid

    #215389

    Purpose

    High expression of the Clusterin(S161R) mutant for purification.

    Depositor

    Franz-Ulrich Hartl

    Insert

    CLU (CLU Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    p.Ser161Arg

    Promoter

    tetO

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GPR150-untagged

    Plasmid

    #233640

    Purpose

    encodes the orphan human GPR150 receptor, untagged

    Depositor

    Christian Gruber

    Insert

    GPR150 (GPR150 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of P418, P423, P425, L426, P427, C430. M…

    Promoter

    CMV

    Available Since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge