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pGMCKq26-10M-hyg

Plasmid

#27337

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

tetR(sB), hygR

Use

Gateway destination vector

Mutation

N/A

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pNX3c2-HA3

Plasmid

#78392

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
natCX

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNR6

Plasmid

#21677

Depositor

James Haber

Article

Rudin et al Mol Cell Biol. 1988 Sep . 8(9):3918-28.

Insert

Ura3 (URA3 Budding Yeast)

Use

Integration sequence

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pHAC111-LST8 (pSW75)

Plasmid

#39211

Depositor

Michael Hall

Article

Wullschleger et al J Biol Chem. 2005 Sep 2;280(35):30697-70

Insert

LST8 (LST8 Budding Yeast)

Expression

Yeast

Promoter

LST8

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
pBN27

Plasmid

#35120

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

fcy1delta1

Use

Yeast replicating

Mutation

amplicon has a complete deletion of FCY1 ORF, fla…

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-puro

Plasmid

#45561

Depositor

Michael McVoy

Article

Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

Insert

Puromycin N-acetyl transferase

Use

Bifunctional selection protein

Tags

EGFP

Expression

Mammalian

Promoter

Phcmv (Strong human cytomegalovirus immediate ear…

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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7TGP

Plasmid

#24305

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-eGFP // SV40-PuroR

Use

Lentiviral

Mutation

EGFP is GFP with S65T and F64L mutations

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pCYP29

Plasmid

#212862

Purpose

Expresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP58

Plasmid

#212891

Purpose

Expresses the Streptomyces griseolus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP24

Plasmid

#212857

Purpose

Expresses the Streptomyces cyslabdanicus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP63

Plasmid

#212896

Purpose

Expresses the Streptomyces clavuligerus LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP33

Plasmid

#212866

Purpose

Expresses the Streptomyces platensis LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCYP25

Plasmid

#212858

Purpose

Expresses the Streptomyces sp. HB202 LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Expression

Bacterial

Promoter

PcymRC

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJC20Tpm1

Plasmid

#99355

Purpose

Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm1

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

Tropomyosin (TPM1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pALACE

Plasmid

#31853

Depositor

Yossef Av-Gay

Article

Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

Type

Empty backbone

Use

Escherichia coli-mycobacterium shuttle plasmid

Tags

6xHIS

Expression

Bacterial

Promoter

Acetamidase operone

Available Since

July 9, 2012

Availability

Academic Institutions and Nonprofits only
pKmK.M1

Plasmid

#125058

Purpose

Storage plasmid for selection marker

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

ScTRP1 marker

Use

Synthetic Biology

Expression

Yeast

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-Neo

Plasmid

#48993

Purpose

Gateway adapted transfer of Neomycin resistance gene

Depositor

Rick Tarleton

Article

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

aminoglycoside 3' phosphotransferase

Use

Gateway attb1 & attb2

Available Since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCB301TOR-GFP

Plasmid

#164799

Purpose

Expression of GFP in oomycetes using Agrobacterium-mediated transformation

Depositor

Miaoying Tian

Article

Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

Insert

GFP

Use

Oomycete expression

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pYS1

Plasmid

#117488

Purpose

Generation of unmarked gene deletions in Mycobacterium smegmatis

Depositor

Eyal Gur

Article

Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

Insert

Che9c 60–61, sacB, aph

Use

Deletion generation

Promoter

Acetamide

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-his3-P41nmt1-3FLAG

Plasmid

#19340

Depositor

Eishi Noguchi

Article

Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

Insert

his3 (his3 Fission Yeast)

Expression

Yeast

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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