We narrowed to 7,526 results for: Ski;

1. pOT_1 - lenti-EFS-LTBR-2A-puro

   Plasmid

   #181970

   Purpose

   Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   LTBR (LTBR Human)
   

   Use

   Lentiviral

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET32a-hEGF

   Plasmid

   #199231

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Epidermal growth factor, partial (EGF Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABEpuro-gateway

   Plasmid

   #51070

   Purpose

   gateway adapted mammalian expression vector

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-TRE-DIO-ChR2-EYFP

   Plasmid

   #183765

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The ChR2-EYFP expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene -teto promoter-flex/dio-ChR2-EYFP
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   ChR2-EYFP

   Expression

   Mammalian

   Promoter

   tetO promoter

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-DIO-hM4Di-mCherry

   Plasmid

   #182532

   Purpose

   This plasmid is for use with neuronal cell-type selective activity tagging. The hM4Di receptor expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene - hSyn promoter-tet-flex-hM4Di-cherry
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   hM4Di-mCherry

   Expression

   Mammalian

   Promoter

   tetO promoter

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIBN-EYFP-hPMLIII

   Plasmid

   #103808

   Purpose

   BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-hPMLIII (CIB1, PML Mustard Weed, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139090

   Purpose

   Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEND-351_Cacnes_dCas9_CRISPRi

   Plasmid

   #225617

   Purpose

   Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. Clone 16 - 2 HH06 pFUSE-IgG1-Knob-mutation knob-clone6

   Plasmid

   #192184

   Purpose

   Clone 16 (HH06) pFUSE-IgG1-Knob-mutation knob-clone6

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 6 heavy chain (HH06)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Clone 16 - 1 HH02 pFUSE-IgG1-Hole mutation-hole-clone2

    Plasmid

    #192183

    Purpose

    Clone 16 (HH02) pFUSE-IgG1-Hole mutation-hole-clone2

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 heavy chain (HH02)
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgGFP_3

    Plasmid

    #78165

    Purpose

    sgGFP

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pPN066

    Plasmid

    #91601

    Purpose

    Express sgRNA targeting human DPP4

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DPP4 (DPP4 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMarsL274G

    Plasmid

    #63177

    Purpose

    This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

    Depositor

    David Tirrell

    Article

    Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

    Insert

    MarsL274G (Mars1 Mouse)
    

    Expression

    Mammalian

    Mutation

    L274G

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYX233-DHFR

    Plasmid

    #163761

    Purpose

    Gal-inducible yeast expression of cytosolic DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    DHFR (Dhfr Mouse)
    

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX Emt-SH2

    Plasmid

    #46424

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Emt (ITK Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 223-346

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR-AmCyan

    Plasmid

    #138481

    Purpose

    For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    AmCyan
    

    Expression

    Mammalian

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX311 MCL1

    Plasmid

    #117729

    Purpose

    Open reading frame vector encoding MCL1

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    MCL1L (MCL1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR v2 sgKEAP1-3

    Plasmid

    #186837

    Purpose

    knock out KEAP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

    Insert

    Keap1 (Kelch-like ECH-associated protein 1) (KEAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA6-N-Myc-SCAP

    Plasmid

    #134285

    Purpose

    Expresses Myc-tagged SCAP in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SCAP (Scap, Scap Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR v2-sgFSP1

    Plasmid

    #186026

    Purpose

    knock out FSP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Ferroptosis Suppressor Protein 1 (AIFM2 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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