Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 5,650 results for: pCas

Showing: 4961 - 4980 of 5650 results

  1. IL1RN gRNA2

    Plasmid
    #113131
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113131-plasmid-map-sequence-id-224942

    Enlarge

  7. Td-CGBE

    Plasmid
    #196599
    Purpose
    Mammalian expression of Td-CGBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    Use
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L); S. pyogenes Cas9 (D10A)
    Promoter
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196599-plasmid-map-sequence-id-386792

    Enlarge

  8. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Article
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

    Enlarge

  9. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62200-plasmid-map-sequence-id-395472

    Enlarge

  11. pCRISPR-cBEST

    Plasmid
    #125689
    Purpose
    C to T base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Insert
    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    ermE*/tipA
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    125689-plasmid-map-sequence-id-271202

    Enlarge

  15. pDGB3alpha2_35S:P19:Tnos (GB1203)

    Plasmid
    #68214
    Purpose
    Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    P19 silencing suppressor
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68214-plasmid-map-sequence-id-119066

    Enlarge

  16. pRvCAHS3-CAHS3-mEGFP

    Plasmid
    #205006
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    RvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205006-plasmid-map-sequence-id-405247

    Enlarge

  17. pRvTUB-mEGFP-NLS

    Plasmid
    #205011
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    mEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205011-plasmid-map-sequence-id-405254

    Enlarge

  18. pRvSAHS1-SAHS1-mEGFP

    Plasmid
    #205007
    Purpose
    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.
    Depositor
    Kazuharu Arakawa
    Article
    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12
    Insert
    RvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
    Use
    Tardigrade expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205007-plasmid-map-sequence-id-405248

    Enlarge

Showing: 4961 - 4980 of 5650 results